Organic Solvent Effect On The Silica Gel Column Separation Of Ginsenoside Rh₂and Rh₃from Enzyme Reaction

Ginsenoside Rh2and Rh3have the function of resisting cancer. The content of these rare ginsenoside in traditional ginseng was limited. The article is about how to use the method of silica gel column to dissociate the mixture of rare ginsenoside Rh2and Rh3which we get them by enzyme hydrolysis from protopanaxadiol type ginsenoside. Basing on the method of using chloroform and methanol as solvent. We try to use dichloromethane and methanol as solvent.Conclusion:we used the proportion of eluant V(chloroform):V(methanol)=9.5:0.5, dissociated the mixture of rare ginsenoside Rh2and Rh3about30gram, we can get Rh2component was about6.36gram, the yield of the Rh2was about21.2%; Rh3component was about2.03gram, the yield of the Rh3was about6.77%. The Rh2component contains20(S)-Rh2and20(R)-Rh2. The Rh3component was the mixture of Rh3, also some other substances.In order to obtain pure Rh3, we used the proportion of eluant V(dichloromethane):V(methanol)=7:0.10、7:0.13、7:0.15、7:0.18、7:0.2、7:0.3to dissociate the mixture of rare ginsenoside Rh2and Rh3about10gram, when the proportion of eluant using V(dichloromethane):V(methanol)=7:0.3, ginsenoside Rh2component and Rh3component were obtained, Rh2component about2.47gram, the yield of the Rh2was about24.7%, HPLC detection showed ginsenoside Rh2was the mixture of20(S)-Rh2and20(R)-Rh2, the purity of ginsenoside Rh2was over90%; Rh3component was about0.41gram, the yield of the Rh3was about4.1%, HPLC detection also showed ginsenoside Rh3was the mixture of cis20,22carbon double bond Rh3and trans20,22carbon double bond Rh3, the purity of ginsenoside Rh3was over90%.In the two methods, the yield of the Rh2dissociated by chloroform and methanol as solvent was high, but not get pure Rh3;the yield of the Rh2dissociated by dichloromethane and methanol was high, the yield of the Rh3was low, but can get pure Rh3successfully:the mixture of cis Rh3and trans Rh3. Provide the basis for industrial production.