Seperation And Purification Of Minor Ginsenoside Compound-K From Enzymatic Product

Ginsenoside Compound K (C-K) is not the natural structure existing in ginseng plant. After ginseng was taken orally, the protopanaxadiol (PPD) type ginsenosides such as Rbl, Rb2, Rc and Rd were hydrolyzed into the active forms of minor ginsenosides (C-K) in human intestinal tract by intestinal bacteria and were subsequently absorbed. Ginsenoside C-K has very good pharmacological and physiological activities, i.e., anticancer, immunomodulatory, anti-inflammatory, therefore obtaining higher purity ginsenoside C-K is important.The conversion of ginsenoside C-K using PPD as substrate by the ginsenosidase producing from sp.848strain was fairly good, the yield of ginsenoside C-K was80%, the yield of ginsenoside F2was10%. The optimal reacted conditions of the ginsenosidase from sp.848strain were:reaction time was24h, the reaction temperature was40℃, the pH was5.0, the substrate concentration was4%.Comparing the purification of ginsenoside C-K by the macroporous absorbitive resin and the organic solvent, it was shown that petroleum ether was better than AB-8resin. The remowing rate of impurity in enzymatic ginsenoside C-K was4.74%by AB-8resin; the petroleum ether was15%.Silica gel column was used to purify the enzymatic product ginsenoside C-K. The result was that the optimal eluted solvent was chloroform:methanol9.5:0.5(v/v), the yield of purified ginsenoside C-K was37.5%, the purity was82.95%and98.30%before and after crystallization detecting by HPLC.