Effectes Of Pinus Massoniana Pollen Extract On Nonalcoholic Fatty Liver In Mice

Modern society human diet high fat, high calorie, sedentary lifestyle leads tononalcoholic fatty liver disease (NAFLD) incidence rate increased significantly, hasbecome an important public health problem. Pine pollen as a food and medicine usedalong with pollen species, for a variety of human diseases prevention and treatmenteffects, including a protective effect on the liver. However, the specific preventionmechanism is not clear. In this study, a mouse model of NAFLD discuss evaluationmasson pine pollen extract (masson pine pollen extract, MPPE) the effect of theprevention and treatment of non-alcoholic fatty liver disease, and to explore themechanism of action of pine pollen as a drug candidate for treating fatty liver orrelated health care products to provide a theoretical basis. This research includes thefollowing three parts.1. MPPE a preventive effect on NAFLD mice：Mice in three MPPE groupsadministrated MPPE by gavage for two weeks in advance. Then treat with high fatdiet for6weeks while giving MPPE, totaling eight weeks. Mouse urine was collectedfor metabolomic analysis. After the end of the experiment, mice were sacrificed tomeasure relevant indicators. Make liver paraffin section, observed pathologicalchanges.The results showed that: compared with the model group, MPPE decreasedserum TG, TC, LDL-C, AST, ALT and liver homogenate MDA levels, elevatedHDL-C levels and increased SOD, GSH activity. Hematoxylin-eosin staining showedthat MPPE can significantly improve the fat diet causes inflammation and necrosis inmice. After the pine pollen prevention, urine metabolism in mice began to improve,there is a tendency to close the control group.2. Therapeutic effect of MPPE on NAFLD mice: Mice fed a high-fat diet for sixweeks to induce fatty liver in mice, and then treated with different doses of masson pine pollen extract for three weeks, totaling nine weeks. Determination of indicators isconsistent with prevention. The results showed that: compared with the model group,MPPE decreased serum TG, TC, LDL-C, AST, ALT and liver homogenate MDAlevels, elevated HDL-C levels and increased SOD, GSH activity. The results ofpathology show that MPPE can significantly reduce the pathological damage of liver,and can reduce the fatty degeneration and inflammation of liver cells. The results ofmetabolic studies showed that the metabolites of urine in mice had changed, and theeffect of high-dose MPPE was the best..3. Effects of MPPE and two kinds of compound in NAFLD mice:Feeding highfat diet for6weeks to establish nonalcoholic fatty liver model, from the beginning ofseventh week group MPPE and compound groups were treated with correspondingdose of the test substance. Hugan tables group was given Hugan tables. Model groupand normal control group were given with an equal volume of normal saline once aday, continued for3weeks. The results showed that: compared with the model group,MPPE decreased serum TG, TC, LDL-C, AST, ALT and liver homogenate MDAlevels, elevated HDL-C levels and increased SOD activity. NAFLD mice after MPPEand two compound intervention, liver damage have been improvement, however,between MPPE and LZ, WWZ compound the results show no significant difference.In short, MPPE has a significant protective effect on mice liver injury induced byhigh fat diet. And the mechanism may be related to anti-free radical lipid peroxidation,regulate lipid metabolism disorders. But the component of MPPE is unclear, need tobe further analysis and research.