The Experimental Study Of Early Enteral Nutrition On Ulcerative Colitis Rats Induced By TNBS

Objective: 1.To investigate the effect of early enteral nutrition in the treatment of ulcerative colitis in rats.2.To investigate whether there is synergistic effect when enteral nutrition combined with SASP in the treatment of ulcerative colitis in rats. 3. To explore the mechanism of enteral nutrition in the treatment of ulcerative colitisMethods: Among the 50 male and female SD rats, 10 rats were randomly selected as the normal control group,and the rest of 40 rats were used the TNBS to induce the rats models of ulcerative colitis.After the succss of modeling by TNBS, the rest of 40 rats were randomly divided into 4 groups: model control group, SASP group, enteral nutrition group,enteral nutrition and SASP group(combined treatment group).The normal control group and the model control group received 0.9% saline gavage, while the enteral nutrition group,SASP group and the combination group were given enteral nutrition, SASP,SASP with enteral nutrition respetively.For all the 50 SD rats,the body weight,clinical symptoms, charateristics of stool,and the blood in the stools were observed everyday to calculation the disease activity index(DAI).After 1 weeks, all the rats were received ceasarean operation after anesthetized, then continue to determine the IFN-γ、TNF-α in the serum of rats by ELISA method.The rats killed after the colon tissue isolation, and take part in colonic mucosa of CMDI score, HE staining and pathological section,All the experimental data were anlyzed by SPSS17.0 statistical software.Results: 1.Compared with the normal control group, the DAI value,CMDI score, serum IFN-γ 、 TNF-α levels were significantly higher in model control group, there are significant differences(P<0.05);2.Compared with the model control group, both enteral nutrition group, combined treatment group and SASP group were able to improve the general state of rats, and reduce the score of CMDI and DAI value, reducing the rat serum IFN-γ、TNF-α, there are significant differences(P<0.05);3. Compared with the enteral nutrition group, the combined treatment group can reduce the CMDI score and DAI value, the serum IF IFN-γ 、 TNF-αt, there are significant differences(P<0.05);4.Compared with the SASP group, the combined treatment group can reduce the CMDI score and DAI value, the serum IF IFN-γ、TNF-αt, there are significant differences(P<0.05);5. Compared with the enteral nutrition group and SASP group, there is no significant difference in serum IFN-γ、TNF-α, CMDI score and DAI value(P>0.05).Conclusion: 1.Through the TNBS modeling method can successfully establish the rat model of ulcerative colitis.2. The body caused by the imbalance of immune regulation of serum IFN- and TNF- increased the content of gamma alpha, is one of the possible mechanisms of ulcerative colitis disease.3.Enteral nutrition on ulcerative colitis have a certain therapeutic effect.4. Enteral nutrition combined with SASP therapy in the treatment of ulcerative colitis rats hadsynergistic effect.