Regionalized Posterior Surface Modification Of IOL And Its Effect On The Migration Of LECs

Objective:To fabricate TGF-β2antibody (anti-TGF-β2) multilayers on the central area of posterior surface of intraocular lens (IOL) by atmospheric pressure glow discharge (APGD) equipment and layer-by-layer self-assembly (LBL) technique, and construct a highly adhesive circular area to form the regional-modified IOL. Test the physical, chemical and biological properties of the regional-modified IOL.Methods:The posterior surface of IOL was pretreated with APGD equipment. Then followed the LBL technique, polyethylenimine (PEI) was deposited onto the center of the posterior surface of IOL to form a circle with a diameter of4mm, followed by TGF-β2antibody and poly L-lysine (PLL), which were sequentially deposited on to the same area for4repetition. At last another TGF-β2antibody layer was deposited on to the same area. The regional-modified IOL was prepared after the multilayers of PEI-(anti-TGF-p2/PLL)4-(anti-TGF-β2) was completed in the central area while the marginal area was left without TGF-β2antibody. Quartz crystal microbalance (QCM) was used to simulate and monitor the assembly process of the TGF-β2antibody multilayers. Atom force microscopy (AFM) and field emission scanning electron microscopy (FESEM) were used to observe the surface morphology. Immunofluorescence and laser scanning confocal microscopy (LSCM) were used to test the immunological activity of the deposited TGF-β2antibody. Cytology experiment was used to test the effect of the regional-modified IOL on the migration of lens epithelial cells.Results:QCM showed a linear weight growth when TGF-β2antibody and PLL sequentially deposited onto the electrode surface. AFM and FESEM examinations showed no significant differences between the regional-modified IOL and untreated IOL. Immunofluorescence and LSCM revealed that TGF-β2antibody was successfully deposited onto the center of the posterior surface of IOL and maintained good immunological activity. No TGF-β2antibody deposited onto the marginal area of the posterior surface of the IOL. Cell scratch assay revealed that the modification of IOL decreased the migration of LECs.Conclusions:The regional-modified IOL was firstly prepared in this study. TGF-P2antibody multilayers was firstly fabricated onto the central area of posterior surface of IOL via APGD plasma pretreatment and LBL technique while the marginal area remained as a highly adhesive surface. The surface morphology remained the same with the untreated IOL. The TGF-β2antibody deposited onto the IOL showed good immunological activity, and could inhibit the migration of LECs in vitro.