Change Of Plasma Cytokine Levels And Their Significance In Children With Different Types Of Henoch-Schonlein Purpura

Objective:The plasma levels of IL-6、IL-17、IL-23、IL-8、 TNF-α and IFN-γ were detected in children with different types of Henoch-Schonlein purpura by enzyme-linked immunosorbent assay (ELISA) method in order to understanding the relationship between cytokines and the pathogenesis of Henoch-Schonlein purpura(HSP).Methods:The blood specimens of 30 normal children group and 180 children with HSP groups were collected. Children with HSP groups included 30 children at acute stage and 30 ones at convalescence stage of primary abdominal type in children with HSP, and 30 children at acute stage and 30 ones at convalescence stage of primary non-abdominal type in children with HSP, and 30 children at their acute stage of secondary abdominal type in children with HSP, and 30 children at their acute stage of secondary non-abdominal type in children with HSP. The plasma levels of IL-6、IL-8、 IL-17、IL-23、TNF-α and IFN-γ were measured by ELISA method for comparison and analyses in normal children group and children with HSP groups.Resμlts:The plasma levels of IL-6、IL-8、IL-17、IL-23 and TNF-α were higher in children with HSP groups than ones in the normal group(P< 0.05), the levels were generally higher at acute stage than at convalescence stage in children with HSP groups(P< 0.05), the levels were also higher in the primary group than on the recurrent group in children with HSP groups(P< 0.05), Comparison of IL-6、 IL-8、IL-17、IL-23 and TNF-α between abdominal type and the non-abdominal type had no statistically significant difference (P> 0.05). The plasma level of IFN-γ was lower in children with HSP groups than ones in the normal group(P< 0.05), the level of IFN-γ was generally lower at acute stage than at convalescence stage in children with HSP groups(P< 0.05), the level of IFN-γ was also lower in the primary group than in the secondary group in children with HSP groups(P< 0.05), Comparison of IFN-γbetween abdominal type and the non-abdominal type had no statistically significant difference (P> 0.05).Conclusions:1.The plasma levels of IL-6、IL-8、IL-17、IL-23、TNF-α are higher in children with HSP than in normal children, and the levels was generally higher at acute stage than at convalescence stage, and the level was also higher in primary group than in secondary group. However, the plasma level of of IFN-γ is lower in children with HSP than in normal children, and the level is generally lower at acute stage than at convalescence stage, and the level was also lower in primary group than in secondary group. These suggeste that the changes of cytokines are associated with the pathogenesis and prognosis of Henoch-Schonlein purpura.2.The comparison of the level of IL-6、IL-8、IL-17、IL-23、 TNF-α and IFN-γ between abdominal type and the non-abdominal type in children with HSP, has no difference, and require further researches.