Histologic Evidence Of Heparananse-induced Portal Vein Tumor Thrombus In Hepatocellμlar Carcinoma

Objective: To seek the histologic evidence of heparananse-induced portal vein tumor thrombus in hepatocellμLar carcinoma. Methods: 1)HPSE expressions in HC cell lines(HCCLM3,HepG2,and LO-2)were measured by real-time reverse transcriptase-polymerase chain reaction(RT-PCR), Western blot analysis and immunohistochemical staining. 2)Establish subcutaneous hepatocarcinoma models on nude mice, study on proliferation and invasion capacity of hepatoma carcinoma cells HCCLM3 and HepG2. 3) Discuss the influence of different doses of heparin sodium on proliferation and invasion capacity of HCCLM3 and HepG2. 4)Establish portal vein aneurysm and thrombus models on nude mice, analysis the formation ability and characteristic of vein aneurysm and thrombus. 5) Discuss the influence of different doses of heparin sodium on formation ability and characteristic of vein aneurysm and thrombus caused by HCCLM3 and HepG2.Results: 1)Both HPSE mRNA and protein relative expression levels were higher in the two types of HC cells than those in normal hepatocyte(P<0.05).HPSE had the highst expression level in HCCLM3 cells. 2) When the concentration is 500 IU, 1 times / every 3 days of heparin sodium injection inoculation concentration cancer cell(HCCLM3 or HepG2,concentration of 3*106/300μL)in nude mice by subcutaneous inoculation of interference of heparanase activity, subcutaneous tumor was necrosis in all or observed as congestion mass after 5 weeks. In comparison, in physiological saline injection group, nude mice vaccinated with cell line HCCLM3 and hepG2, HCCLM3 subcutaneous mass was significantly bigger than HepG2 inoculation one(P<0.01). 3) After 5 weeks, inject in nude mice Lower abdominal peritoneal with HCCLM3 cell( concentration of 3*106/300μL), it indicated that the tumor liver metastasis rate was 100% and the portal vein tumor thrombus formation rate was 40%. As contract, inject in nude mice Lower abdominal peritoneal with HepG2 cell( concentration of 3*106/300μL), it indicated that the tumor liver metastasis rate was 40% and the tumor of liver metastasis rate was 0%. 4) Inoculation with concentration of 3*106/300μL tumor cells after 1 weeks, subcutaneous injection concentration was 500 IU, 1 times / 3 days, no liver tumors formed laterly; nude mice intraperitoneal inoculated with 3*106/300μL HepG2, no portal cancerous thrombus and liver metastases block formed.Conclusion: 1) Carcinoma cells(HCCLM3) has characteristics of high heparanase gene and protein expression. 2) In nude mice subcutaneous tumor model, tumor formation rate and survival rate are high, which could be used for the proliferation of tumor invasion research. 3) Heparin can obviously inhibit the high heparanase express on liver carcinoma cell proliferation, invasion activity. 4) The success on establishing nude mice liver tumor and portal vein model indicating lower abdomen injection has certain significance on studies of tumor metastasis. 5) Heparin caninhibit the heparanase’s high expression on hepatocellular carcinoma become tumor and metastases ability of portal vein tumor and thrombus. It shows heparanasein hepatocarcinoma high expression may be associated with tumor cell proliferation, invasion, metastasis, which is worthy of further study.