| Purpose:This paper aims to study animal experiments, observe the effects of kidney and spleen solid red square endometrial hyperplasia model of ovariectomized rats vasomotor function, and to further explore the role of kidney and spleen solid red square from the uterine microenvironment vasoactive substances mechanism for the treatment of adolescent DUB provide a broader basis for scientific thinking and clinical treatment.Methods:In this experiment, juvenile female SD rats were 90 for the study, ovariectomized estrogen stimulation of endometrial hyperplasia build models to estradiol valerate, kidney and spleen solid red square as the experimental medication. 30 days in rats, weighing 90 ± 10 g line ovariectomized surgery, for 3 consecutive days by intraperitoneal injection of sodium penicillin 200,000 units, five days vaginal smear, vaginal exfoliated cells under the microscope, no keratinocytes showed that castration success. Ovariectomized rats intragastrically successful estradiol valerate 45 days, the establishment of endometrial hyperplasia in rat models. The model was successful models were randomly divided into five groups, namely solid red kidney and spleen high dose group, kidney and spleen solid red square low-dose group, Gongxueting group, medroxyprogesterone acetate group, model group. Under the same kinds of rearing conditions, cage feeding, timing gavage once daily.Kidney and spleen solid punch to the side of the high-dose group were solid red kidney and spleen high dose 15 g / kg(crude drug concentration of about 1.5g / ml), equivalent to 6.7 times the clinical dose, kidney and spleen solid punch to the side of the low-dose group were healthy kidney spleen Guchong low dosage 7.5g / kg(crude drug concentration of about 0.75 g / ml), equivalent to 3.35 times the clinical dose, Gongxueting group fed with Gongxueting suspension 12 g / kg(concentration of about 0.6g / ml), medroxyprogesterone acetate group fed with medroxyprogesterone acetate suspension 2mg / kg(concentration of about 0.1mg / ml), model control group fed with an equal volume of water, each group were sacrificed after 30 days of continuous perfusion large mice were measured indicators. By enzyme-linked immunosorbent assay in the serum nitric oxide(NO), endothelin-1(ET-1), TXB2,6-Keto-PGF1α levels, enzyme-linked immunosorbent assay uterus homogenates prostaglandin F2α(PGF2α), prostaglandin E2(PGE2), nitric oxide(NO), endothelin-1(ET-1) levels. Statistical analysis All data are realized under the support of SPSS18.0 software.Results:(1) can the solid red kidney and spleen and uterine serum levels of NO decreased(P <0.05), significantly increased(P <0.01) ET-1 serum and uterus, ET-1 / NO ratio was significantly increased(P <0.01);(2) solid red ki dney and spleen homogenates of rat uterine side of PGE2 was significantly lo wer(P <0.05), homogenized content PGF2α significantly increased(P <0.05), PGF2α / PGE2 the ratio was significantly higher(P <0.01);(3)solid red kidne y and spleen can the significant increase in serum TXB2 content(P <0.01), s erum 6-Keto-PGF1α was significantly lower(P <0.05), TXB2/6-Keto-PGF1α w ere significantly increased(P<0.01).Conclusion:Solid red kidney and spleen can only improve the level of ju venile uterine endometrial hyperplasia model ovariectomized rat uterus and ser um ET enhance uterine local vasoconstriction; while reducing uterine local an d serum NO levels decreased uterine local vasodilation. Meanwhile, kidney an d spleen solid red square can significantly regulate the content of the rat uter us and serum prostaglandin, the ratio between the balance of prostaglandins, s uggesting solid red kidney and spleen can only regulate endometrial hyperplas ia in young ovariectomized rats model of uterine vascular microenvironment v asomotor system level, to promote coordination of endometrial vasomotor func tion, effectively improve the local micro-environment of the uterus, kidney an d spleen for clinical use solid punch treatment of adolescent DUB provide an experimental basis. |
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