Coptis Chinensis And Its Processed Products And The Material Basis Of Comparative Study On The Antibacterial Spectrum

Coptis chinensis is a traditional common qingrejiedu medicine, living with their more very, easy injury taste, not easy long suit. Clinically, according to the different conditions, have a variety of coptis chinensis processed products. The processing types of rhizoma coptidis is various, processed products in past dynasties literature more than 24. The wine rhizoma coptidis rhizoma coptidis, turmeric, calling has been in use today, 2010 edition of pharmacopoeia records.Rhizoma coptidis after ginger system can ease their understanding of sex, and good stomach fire vomiting. Coptis chinensis as heat purging fire to medicine, ginger warm stomach anti-nausea, the effective medicine for ou home, with ginger Zhi Huang even, can ease coptis sexual experience, and the fire of stomach heat, take down inverse of ginger, medicine into the rear not back out.The method of main is according to the 2010 edition pharmacopeia ginger, rhizoma coptidis with ginger juice. Mix well and set the pot, gently stir until ginger sucked, remove and dry.Diacolation method is adopted, with orthogonal experiment design, the coptis and the extract of curcuma, screening in solvent dosage, dipping time, velocity and concentration of ethanol into four factors. To extract yield, the main alkaloids and total alkali content, antibacterial effect as examining index, comprehensive evaluation results, select the best extraction process. Results: the dipping time for 18 hours, six times the amount of solvent, solvent concentration was 70% ethanol solution influence factor has no significant difference(velocity).To identify rhizoma coptidis, turmeric differences, even in the main alkaloids content of coptis chinensis and turmeric even the main chemical components were analyzed. Turmeric raw rhizoma coptidis alcohol extraction part even after five alkaloids increase: 114.94%, 130.56%, 62.15%, 164.23%, 62.15%, methylene chloride parts: 28.74%, 87.88%, 7.89%, 18.22%, 7.89%, ethyl acetate parts: 72.16%, 266.67%, 160.92%, 128.74%, 160.92%, n-butanol part: 18.43%, 13.04%, 5.68%, 64.42%, 5.68%; Ethyl acetate parts of increase is especially significant, table berberine and hydrastine, Martin, berberine were increased by more than 100%, table berberine has increased 266.67%.For coptis root and turmeric features, even the whole composition differences in the study of rhizoma coptidis and turmeric even HPLC feature maps. By means of both HPLC analysis, and comparison, rhizoma coptidis, turmeric has 14 common peak, calibration of four peaks are respectively table berberine and hydrastine, Martin, berberine hydrochloride, but the content of raw rhizoma coptidis were increased. After feature the maps of rhizoma coptidis yinpian and turmeric even the main alkaloids chemical constituents content analysis found that 12 and 13, 13 and 14, the peak area ratio in a significant change before and after processing. Born in rhizoma coptidis yinpian, 12 and 13 peak area ratio less than 1, while turmeric even greater than 1; Raw rhizoma coptidis 13 and 14, the peak area ratio greater than 3 and turmeric even less than 3. To identify rhizoma coptidis, turmeric advantage of antimicrobial spectrum, according to the Chinese goldthread attending functions, chose the klebsiella pneumoniae, ordinary proteus, singular deformation coli, pseudomonas aeruginosa, staphylococcus aureus, escherichia coli strains. Compared through the antibacterial experiment, the results show that: total coptis extract parts of pseudomonas aeruginosa, singular proteus, ordinary proteus no antibacterial effect, but turmeric even for the three strains of antimicrobial effect obviously, especially for pseudomonas aeruginosa has strong antibacterial effect. For the analysis of the differences of the extraction parts further study, found that raw rhizoma coptidis methylene chloride parts of klebsiella pneumoniae bacterium, ordinary singular proteus proteus, don’t have antibacterial effect, but turmeric even has an obvious antibacterial effect; Ethyl acetate parts of rhizoma coptidis have no antimicrobial effect of pseudomonas aeruginosa, but turmeric even ethyl acetate parts of pseudomonas aeruginosa is especially significant antibacterial effect; N-butanol part before and after the processing of various strains had antibacterial effect, but turmeric even effect is stronger than raw rhizoma coptidis. Determine headquarters of rhizoma coptidis, turmeric even antibacterial spectrum and influence a key extracted parts of efficacy. To clarify the advantages of each part of the total extract and antibacterial spectrum, according to the Chinese goldthread attending functions, chose the klebsiella pneumoniae, ordinary proteus, singular deformation coli, pseudomonas aeruginosa, staphylococcus aureus, escherichia coli as the antimicrobial object. Through the antibacterial experiment, comparing the different parts of antimicrobial effect, the results showed: total extracted parts, pseudomonas aeruginosa, rhizoma coptidis singular proteus, the general has no effect of bacillus proteus, but of the three kinds of turmeric have antibacterial effect especially the pseudomonas aeruginosa has strong antibacterial effect. The advantage of extracted part antibacterial spectrum, it is found that raw rhizoma coptidis methylene chloride parts of klebsiella pneumoniae bacterium, ordinary singular proteus proteus, no antimicrobial effect, but turmeric even has an obvious antibacterial effect; And turmeric of ethyl acetate parts of pseudomonas aeruginosa is especially significant antibacterial effect; N-butanol part before and after processing has antibacterial effect, but the curcuma effects are stronger than raw rhizoma coptidis. So that rhizoma coptidis after ginger main advantage to increased levels of the main alkaloids determination of antibacterial has obvious effect, and enhanced the antibacterial efficacy.Through the establishment of the chemical component ralationship interpretation from the perspective of material base processing mechanism of rhizoma coptidis, determine its antibacterial effect in clinical. To identify rhizoma coptidis with turmeric differences in ethyl acetate parts of antimicrobial spectrum, analyzing its main alkaloid content than the concentration-response relationship between parts of antimicrobial effect, is to determine the composition of the influence different antibacterial effects than turmeric extract, ethyl acetate parts even ratio is: 7.69:3.35:10.00:17.59:24.42. And through the statistical method of permutation and combination of five kinds of alkaloid composition according to the ratio of C55, find out the relationship of the influence effect of antibacterial effects of alkali and berberine ingredient is medicine.This paper mainly through to coptis root ginger system before and after suction and digestive tract bacteria antibacterial effect difference as an index, analysis of rhizoma coptidis, turmeric advantage antibacterial spectrum. Modern technology is used to analyze the change law of the chemical composition before and after the whole chemical analysis of alkaloid content and ratio of main differences influence on antibacterial effect, processing mechanism to lay the foundation for interpretation and provide good basis for guiding clinical medication.