Expression Of NF-KB、CD10 And CD133 In Endometrial Polyps

Endometrial polyps(EPs) is a common gynecological disease, especially in fertile women and postmenopausal women. The incidence of EPs has a rising trend in recent years. According to some reports in the literature, the prevalence of EPs in our country is as hinging as 24%-25%.The clinical manifestation of patients with endometrial polyps are: menstrual disorders, the amount of menstrual increased, menstrual extension, even worse, it may be has a malignant transformation. At the same time, the overload endometrial polyp in the womb, which obstruct the implantation of sperm and egg and case infertility.Although the tendency of EPs is increased gradually, but the pathogenesis of it has not been clear. The EPs is easy to relapse, but most clinicians have no efficient method to prevent it. For the diagnosis of endometrial polyps most clinicians by the patient’s clinical manifestation and related auxiliary examination, but it often has certain misdiagnosis and missed diagnosis, which not only can influence our country women’s work, but also will seriously affect their daily life. According to statistics, at present the measures of diagnosis the endometrial polyps is as follows: The first measure is transvaginal ultrasound: the two-dimensional image of EPs is more like high echogenic mass of particles or droplets. The internal diameter of EPs is less than 5 mm, generally it has no blood flow signal but the large EPs can have blood flow signals. The second measure is hysteroscopy examination: The performance of EPs in the microscopic is signal or multiple vegetations with soft character、pink color, which diameter is 1-2 mm. The third measure is pathological: the three sides of EPs is backed covere by surface epithelium, at the same time prominent feature of EPs is fibrous stroma and thick wall vessel. But there are still some shortcomings in these auxiliary examination, which can bring certain influence to the clinical diagnosis and treatment of EPs. From the perspective of learning stress of this study, learning the expression of NF-KB、CD10、CD133in endometrial polyps.NF-KB is a nucleoprotein factor, which can combine with a special site of immumoglobulin.NF-KB is a large family,it distributed in a variety of cells, it has a close relationship with the body’s immune response 、inflammation、tumor growth and so on. In recent years, studies have shown that NF-KB have take part in the development of endometriosis、endometrial cancer、nasal polyps and so on, but there are rarely reports prove that NF-KB have a relationship with the development of endometrial polyps. The occurrence of endometrial polyp contains many related factors, and the study shows that the occurrence of EPs may be an inflammatory reaction, so the activation of NF-KB may be involved in the expression of related cytokines of EPs.CD10、CD133is one of the members of Prominin family, is the human five transmembrane glycoprotein, which is homologous with rat Prominin. CD10 were expressed in human endometrial stromal cells, and have no expression in endometrial epithelial cells, so there are studies thought that CD10 can serve as the immune markers for endometrial stromal cells. CD133 is positioned in the cell membrane and cytoplasm, which usually expressed in various tissues of stem or starting cells, is so far the most valuable markers of screening cancer stem, used to separate and identify stem cells in many organizations. The biological function of CD133 is unclear, a study show that it expression in the process of the development of endometrial hyperplasia is showed a trend of gradually increased, considering the increase of its biological activity may promote the proliferation of glandular epithelial by some of the molecular pathways.Objective:Using the detection method of immunohistochemical to detective the expression of nuclear transcription factor KB、cluster of differentiation 10、cluster of differentiation 133 in the group of endometrial polyps and normal endometrium group, discuss the expression and mechanism of NF-KB、CD10、CD133 in the development of endometrial polyps,so as to provide more important reference indexes for the diagnosis of endometrial polyps. If the study have a desired outcome,it can reduce the misdiagnosis rate and the missed diagnosis of endometrial polyps.Methods:Choosing 120 cases of patients who has been confirmed of endometrial polyps by hysteroscopy surgery and pathology in September 2013-May2014 as the experimental group; over the same period selecting 30 number of patients who line with irregular uterine bleeding in our hospital clinical scraping and confirmed normal proliferative endometrium as control group. In the study of the group of endometrial polyps found: the expression of CD133 in specimens with secretory reaction is different,so the experimental group will be divided into the EPs group with secretory of function and the EPs group hyperplasia of reaction. All patients according to diagnostic criteria and inclusion criteria.We obtained specimens under sterile conditions, some tissue was fixed in 10% formalin solution embedded in paraffin, and sliced, which was used finally after pathologically confirmed. Testing the expression of NFKB protein、CD10 pretein and CD133 protein in the experimental group and control group by immunohistochemical SP method. Then analyze the expression of NF-KB 、 CD10 、 CD133 in the two groups by SPSS19.0 software. According to the classification of negative 、 weak positive 、positive and strong positive for data statistics. Finally using the method of chi-square statistical treatment to evaluate the outcome.Results:1 The expression of NF-KB protein in the group of EPs and normal endometrium.The expression of NF-KB in endometrial polyps group and normal endometrium group were both in the cell membrane and cytoplasm, theoutcome of immunohistochemical staining is tan particles. The positive expression rate of NF-KB in EPs is 77.5%,but in normal endometrium group the positive expression rate is 46.67%,so NF-KB is highly expressed in EPs, the difference between the two group has statistical significance(χ2=4.2,P=0.031).2 The expression of CD10 protein in the group of EPs and normal endometrium.CD10 have a positive expression in endometrial stromal cells, according to the statistical date,the positive expression rate of CD10 in EPs is 80%,and in the control group is 96.7%,comparing the two groups the difference between the two group have no statistically significant(χ2=2.9,P=0.087).But the strong positive expression rate in the group of EPS is 7.5%,in control group is 23.3%,there have a statistically significant difference between the two groups(χ2=19.68,P=0.001).3 The expression of CD133 protein in the group of EPs and normal endometrium.The expression of CD133 is in the cell membrane and cytoplasm, CD133 has a positive expression in both of the two group, The positive expression rate of CD133 in the group of EPs is 69.16%,in the group of proliferative endometrium is 26.67%, by the comparison between groups the difference is statistically significant(χ2=17.96,P=0.000).CD133 in the endometrial polyps with secretion the positive expression rate is 83.05%;in the group of EPs with mainly hyperplasia period the positive expression is 55.74%,by the comparison between groups the difference is statistically significant(χ2=10.49,P=0.013).Conclusion:1 NF-KB is highly expressed in endometrial polyps group.2 The strong positive expression of CD10 in endometrial polyps group is lower than the control group.3 The positive expression of CD133 in endomtetrial polyps group is higher the control group. At the same time the positive expression of CD133 in the endometrial polyps with secretion is higher than the group of EPs with mainly hyperplasia period.4 The different expression of NF-KB、CD10 and CD133 may be have a connected with the generation of endometrial polyps.5 The expression of NF-KB、CD10、CD133 in EPs may as an important reference index of diagnosis of endometrial polyps.