Identification Of The Boundaries Of Colorectal And Breast Carcinomas Based On Multiphoton Microscopy

Cancer is one of the leading reasons to death in modern human society. In the overall statistical data of cancers, the incidences and mortalities of colorectal cancer and breast cancer are in the top three. Early detections and timely treatments are very important, however, most of the colorectal cancers and breast cancers are found with having been developed to a malignant stage due to the limitation of ability of current tumor diagnosis technology. Therefore, surgical resection remains being one of the most radical and effectively curative treatments for colorectal cancers and breast cancers. The precise identification of tumor boundary plays a very important role in tumor resection surgery. In recent years, multiphoton microscopy(MPM)are emerging as one of important applications of non-linear optics and NIR microscopy in life sciences. MPM imaging technique bases on two-photon fluorescence excitation(TPEF) and second harmonic generation(SHG), with abundant remarkable advantages such as, low light bleaching and light damage, high resolution and high sensitivity, strong penetrating power, unnecessary fluorescent dyes, non-invasion and providing real-time imaging, which enable MPM to be very suitable for imaging human biological tissues.In this paper, firstly, we use MPM to image the boundary areas between tubular adenocarcinoma and normal rectal mucosa, and then obtain MPM images in two channels respectively. We analyze these digital pathological images in histomorphology views which reveal the detailed informations of microstructures of biological tissues. These features revealed in MPM images are correlated readily with hematoxylin and eosin(H&E) stained images. In the same way, we employ MPM to scan the boundary areas between invasive ductal carcinoma and normal breast tissue, and obtained MPM images in the two channels, of which the results are in accordance with results of pathological H&E stained images. At the mean time,we deal with experimental datas thorough quantitative analysis.Our experiment results show that the MPM as a novel imaging technique for biomedical tissues, can be used for identification of tumor boundaries. In the foreseeable future, miniaturized and integrated MPM equipments will play a huge role in the surgical resection for colorectal cancers and breast cancers.