Chemical Components And Antioxidation Activities Of Essential Oils From Angelica Sinensis Radix,Ligusticum Chuanxiong Rhizoma And Acorus Tatarinowii Rhizoma

The optimization of extracting technology and the inclusion process of β-cyclodextrin (β-CD) complex conditions of the essential oil from Angelica sinensis, Ligusticum Chuanxiong and Acorus tatarinowii were studied. By orthogonal design, volatile oil was obtained by vapor distillation. The extracting technology was optimized with the contents of volatile oil being the optimizing objective. The β-CD complex was made by saturated water solution. The best inclusion technology was selected and complex was tested with inclusion ratio. The inclusion compound was analyzed by TLC, UC and IR. The optimum extracting conditions were represented as follows:crushing the medicinal material to 10 Mu granularity, extracting for 8h without advance dippingin. The inclusion process was that the ratio of β-CD and volatile oil was 8:1(mL:g), inclusion temperature was 60℃ and stirring time was 2h. The optimimum extraction technology was conformed. The inclusion is available and the complex is stable.In this study, volatile oils extracted from Angelica sinensis and Ligusticum Chuanxiongand Acorus tatarinowii were test by gas chromatography-mass spectrometry analysis. In total,22,47 and 28 volatile chemical components in essential oils from Angelica sinensis, Ligusticum Chuanxiong and Acorus tatarinowii were qualitatively and quantitatively determined, representing 92.31%,97.83% and 90.88% of the total content, respectively. Z-ligustilide,3-butylidenephthalide, n-butylphthalide, E-ligustilide and Linoleic acid were found in essential oils from Angelica sinensis. The main compounds in essential oils from Ligusticum Chuanxiong were ligustilide, Terpinen-4-ol, Eudesmene, Espatulenol, 3-butylidenephthalide, Palmitic acid and Linoleic acid. The main compounds of essential oils from Acorus tatarinowii were β-Asarone, Methyl isoeugenol and 4-Allylanisole. The data gathered in this study may be helpful for understanding the synergistic nature of these drugs in traditional Chinese traditional medicine.Meanwhile the antioxidant activities of the essential oils from Angelica sinensis, Ligusticum Chuanxiong and Acorus tatarinowii were studied on DPPH·, ABTS· radical scavenging properties and reducing power and compared with Vc. The results showed that the essential oils from Angelica sinensis, Ligusticum Chuanxiong and Acorus tatarinowii had antioxidative activities depending on the concentrations, and the effect of essential oils from Angelica sinensis was stronger than Ligusticum Chuanxiongand Acorus tatarinowii.Study on the oxidative damages of cell line SH-SY5Y exposed to H2O2 and the protective and prothetic effects of the different essential oils. Human neuroblastoma SH-SY5Y cell was exposed to H2O2 in vitro to establish oxidative injury model. The MTT method was applied to examine the growth of oxidative injury model cell treated with the essential oils from Angelica sinensis, Ligusticum Chuanxiong and Acorus tatarinowii. The results showed that, in a certain concentration range, the essential oils from Angelica sinensis, Ligusticum Chuanxiong and Acorus tatarinowii all were significant protective effects (P<0.05). And in a certain concentration range, the essential oils from Angelica sinensis, Ligusticum Chuanxiong were significant prothetic effects (P<0.05),the essential oils of Acorus tatarinowii was no significant prothetic effects (P>0.05).