Study On The Qams And Specific Chromatograms Of Volatile Oil Of Chuanxiong Rhizoma, Angelicae Sinensis Radix, Ligustici Rhizoma Et Radix

AIM:(1) To establish a HPLC method for determination of senkyunolide A and ligustilide in Chuanxiong Rhizoma, Angelicae Sinensis Radix, Ligustici Rhizoma et Radix, Duliang Dropping Pills, Baidai pill and Xuefuzhuyu Capsules by substitute reference substance. (2) To identify Chuanxiong Rhizoma, Angelicae Sinensis Radix and Ligustici Rhizoma et Radix by establishing the HPLC specific chromatograms of their volatile oil and comparing their specific peaks. Their specific peaks were analysed by HPLC-MS. Methods:(1) To measure the correction factor of ligustilide and senkyunolide A under different conditions using the butylphalide as substitute reference substance, and the ligustilide and senkyunolide A in Chuanxiong Rhizoma, Angelicae Sinensis Radix, Ligustici Rhizoma et Radix, Duliang Dropping Pills, Baidai pill and Xuefuzhuyu Capsules were determined by substitute reference substance and the relative correction factor. (2) The HPLC method was used, and methanol-water (55:45) was used as mobile phase. Similarity evaluation system for chromatographic fingerprint of TCM (2004 AB) was used in data analysis on fingerprints of Chuanxiong Rhizoma, Angelicae Sinensis Radix and Ligustici rhizoma et radix. Their HPLC specific chromatograms were established. Their specific peaks were analysed by HPLC-MS. Results:(1) The linear response ranges (n=6) of butylphalide, senkyunolide A and ligustilide were 0.1994～3.988 μg (r=0.9999), 0.04346～0.8692 μg (r=0.9999) and 0.08067～1.6134 μg (r=0.9999) respectively. Relative correction factors of senkyunolide A and ligustilide with reference to butylphalide were 0.4907 and 0.2263. The relative correction factor has good reproducibility. There is no significant difference between QAMS and external standard method. (2) Under the selected spectrum condition, their HPLC specific chromatograms were established. Senkyunolide A, butylphalide, coniferylferulate, E-ligustilide, Z-ligustilide, neocnidilide and E-butylidenephthalide were identified as specific peaks in chromatograms based on their MS data. Conclusion:The substitute reference substance is adopted to determine senkyunolide A and ligustilide in Chuanxiong Rhizoma, Angelicae Sinensis Radix and Ligustici Rhizoma et Radix. This accurate method is available and applied for quality of Chuanxiong Rhizoma, Angelicae Sinensis Radix, Ligustici rhizoma et radix and Chinese Patent Medicine. The HPLC fingerprint has strong characteristic and specificity, This method can be used to distinguish Chuanxiong Rhizoma from Angelicae Sinensis Radix and Ligustici rhizoma et radix.