Research On Extraction Process And Antioxidant Activities From Total Phenolic Acid Of Blumea Balsamifera(Linn.)DC

Blumea balsamifera DC. also known as winds, ear of moxa, wind leaf, borneol,ai etc., for the compositae plants of the genus einar incense einar incense all the grass,it is li, miao, dai, buyi, aquatic animals, such as the important ethnic minority medicine, has the functions of dehumidification, qingrejiedu, water swelling effect and so on, is used for cold dampness bowel, swelling, bones and muscles pain,diarrhea, abdominal pain, injuries, ringworm sores and other disease treatment,medicine has a long history and unique efficacy, in southeast Asia, India, Singapore,Thailand, Malaysia, Australia and other countries also have Blumea balsamifera DC.incense as a medicine.Its main composition is a lot of scholars both at home and abroad research, including flavonoids ingredients have antitumor activity, antioxidant activity, resistance to tyrosine kinase activity. Although studies have shown that it contains a variety of phenol acid and flavonoids, but its correlation with antioxidant activity has yet to see the detailed report. Now as a result of the synthetic antioxidants growing cancer and harm to the health of the body, natural antioxidant is more and more get the attention of people.For the further development and utilization of Blumea balsamifera DC. antioxidant properties, this study conducted its phenol acid extraction method of optimization;Blumea balsamifera DC. sweet different parts were measured, the content of total polyphenols acid and flavonoids in their oxidation resistance is measured at the same time;Analyses the alcohol extract of antioxidant activity and its correlation of flavonoids, total polyphenol content;For Blumea balsamifera DC. of polyphenols compounds in comprehensive development and utilization to lay the scientific basis.Comprehensive research content of this article,and the experimental results, summarized as follows:1. To establish a determination method of total polyphenol content in Blumeabalsamifera DC. aromatic extract.Using UV, through the stability, precision and reproducibility and the recovery ratio of sample investigation, the results showed that the distilled water as extraction solvent, gallic acid as reference substance, detection wavelength of 760 nm, the reaction time for 30 min quantitative analysis method can fragrance of total polyphenol content in different parts.Through different parts of its incense determination of the content of total polyphenols and flavonoids found that different parts of the content of total polyphenols and flavonoids have obvious difference,respectively is functional leaf > leaves > stems.2. The application of single factor design and response surface method optimization Blumea balsamifera DC. incense polyphenol content extraction process.Of Blumea balsamifera DC. incense antioxidant active ingredient extraction process is optimized, by investigating the water first three, 95% methanol, 75%ethanol extraction solvent, using uv spectrophotometry, determine the water as extraction solvent.Using single factor experiment to determine the extraction time and solid-liquid ratio and extraction power single factor optimal values of three factors,and then through the single factor analysis of variance, multiple factors determine against oxidation active ingredient extraction yield factors that influence the maximum of BBD(Box- Behnken Design) response optimization, so as to determine the optimal combination.The experimental results show that the optimal extraction technology for the extraction of: time for 30 min, material liquid ratio of 1:60, to extract power of 400 W.3. Different tissue parts Blumea balsamifera DC. fragrantthe content of total polyphenols acid and flavonoids in researchUsing DPPH and ABTS free radicals method for determining Blumea balsamifera DC. different parts of the in vitro antioxidant capacity, as well as the determination of flavonoids in different parts, the content of total polyphenols analysis Blumea balsamifera DC. fragrance extracts antioxidant activity and its main chemical composition of the correlation.The experimental results show that Blumea balsamifera DC. incense of total flavonoids and total polyphenol content in differentparts of the difference, respectively is functional leaf > leaves > stems.Under the same mass concentration, and its antioxidant capacity, in turn, are: function leaf > leaves >stems.By analyzing it incense different parts of the content of total polyphenols and flavonoids and antioxidant activity of correlation, it incense ethanol extract of total antioxidant capacity and total flavonoids and total polyphenol content are positively correlated, among them, the total flavonoids and antioxidant activity of the correlation coefficient is higher than the correlation coefficient of polyphenols and antioxidant activity, therefore thinks its total for the main antioxidant activity of flavonoids in the incense effective component, total polyphenol antioxidant activity of the active ingredients.4.Different polar parts Blumea balsamifera DC. fragrant extract antioxidant activity Condensed the Blumea balsamifera DC. incense function leaf ethanol extract, extract with different polarity extraction, followed by petroleum ether, ethyl acetate,n-butanol layer and water layer, using enzyme standard meter measure its ability to remove DPPH and ABTS, FRAP free radicals.The experimental results show that its incense function leaf extract ethyl acetate parts of the highest rates, the DPPH clearance ability is strongest in the extraction of parts.Overall, compared with the ethanol crude extract, after ethyl acetate extraction, the antioxidant activity was strengthened, in addition, different polar solvent extraction, antioxidant activity of different mechanism system appeared in the advantage of different extraction parts, so can according to the requirements of different antioxidant activity, with different polar solvent extraction.