Clinical Research Of Activity Of Blood T Lymphocyte Subsets In Perioperative Lung Cancer Patients

Objectives To investigate the expression of activity of T lymphocyte subset in FB of lung cancer patient during perioperative period; analyze changes of immunologic function of the lung cancer patient during the perioperative period and whether there is correlation between immunologic difference and gender, age, pathological distribution,grade of cell differentiation and TNM staging of these patients; evaluate immunologic function of the patients during their perioperative period and provide clinical analysis to guide postoperative clinical treatment in a bid to offer theoretical support for postoperative relapse or metastasis of the disease.Methods 1 Grouping: We select 30 NSCLC patients with appropriate surgical indications as the research group; we select 20 people coming to the health center for physical examination as the control group. The research group is further divided into the preoperative subset and the postoperative subset; the group of lung cancer patients is divided based on their TNM stages, pathological types, grade of cell differentiation, age and gender. 2 Testing method: Draw 2~3 mls of the fasting peripheral venous blood of cancer patients before the surgery and on Day 1, 3 and 11 after the surgery respectively;draw 2~3 mls of the fasting peripheral venous blood of the control group; all drawn blood samples are collected in the EDTA plain tube before they are test for expression of T lymphocyte activity by means of the FCM method and step. 3 The data obtained in the test are summarized and analyzed with SPSS17.0. The quantitative data of each group are expressed with arithmetic mean±SD(z±s), with the T test conducted for the relevant groups; the statistical difference is considered significant when P<0.05, and very significant when P<0.01.Results 1 The activity expression levels of CD3+/CD45+, CD3+CD4+/CD45+ in PB of patients are lower than the healthy group. However, the activity expression levels of CD3+CD8+/CD45+ of the research group are higher while the ratio of CD3+CD4+/CD3+CD8+ is lower. The difference between the two groups is considered significant(P<0.05). 2 On Day 1 after the surgery, the activity expression levels of T lymphocyte subset CD3+/CD45+, CD3+CD4+/CD45+ in PVB for the test group are further lowered, while the ratio of CD3+CD4+/CD3+CD8+ falls, which is significant when compared to that before the surgery(P<0.05); the difference of activity expression levels of CD3+CD8+/CD45+ is not enough to show statistical significance(P>0.05); the activity expression levels of CD3+/CD45+, CD3+CD4+/CD45+ on Day 3 after the surgery slightly rebound, but is still lower than before, while the change of activity expression of CD3+CD8+/CD45+ is not salient; on Day 11 after the surgery, the activity expression levels of CD3+/CD45+, CD3+CD4+/CD45+ and the ratio of CD3+CD4+/CD3+CD8+ for the test group both rise obviously and show statistical significance compared to those valuesbefore the surgery(P<0.05),close to but still lower than the control group; the activity expression levels of CD3+CD8+/CD45+ drops and the difference is considered significant(P<0.05). 3 We conduct analysis for the patients by their subsets based on their pathological patterns, grades of cell differentiation, TNM staging, gender and age; the result shows that the activity expression of CD3+/CD45+, CD3+CD4+/CD45+ for the patients increases with the rise of TNM stages and decreases with the fall of grades of pathological differentiation. When comparing patients of Stage Ⅲ and Stage Ⅰ lung cancer, we find that the activity expression levels of CD3+/CD45+ and CD3+CD4+/CD45+for the former drop more obviously(P>0.05); there is no significant difference of the activity expression levels of CD3+/CD45+, CD3+CD4+/CD45+ and CD3+CD8+/CD45+between patients of Stage Ⅰ and Ⅱ lung cancer; significance is observed in terms of activity expression levels of CD3+/CD45+, CD3+CD4+/CD45+and CD3+CD8+/CD45+between the poorly and highly differentiated subsets(P<0.05); no statistical difference is seen regarding expression levels of CD3+/CD45+, CD3+CD4+/CD45+ and CD3+CD8+/CD45+between patients of pathological patterns, gender and age before and after the surgery(P>0.05).Conclusions 1 The activites expression levels of T lymphocyte CD3+/CD45+,CD3+CD4+/CD45+ratio decrease, the CD3+CD8+/CD45+ratio increase, according to their immune functions. 2 T lymphocyte subsets CD3+/CD45+ and CD3+CD4+/CD45+activities ratio increases in patients with lung cancer, the CD3+CD8+/CD45+ ratio decline,indicating the immune function gradually restore, speculating that athe decrease in immune function of patients with lung cancer is caused by reason of sex with tumor immunosuppression. 3 Differences in immune function of patients with lung cancer may correlate with tumor differentiation, and TNM staging, pathological type, age, gender,and tumor may not related. Postoperative(11 days) of the immune status recovered,different pathological type, degree of cell differentiation, TNM stage, have no obvious difference between gender and age. 4 Detection of lung cancer patients perioperative of peripheral blood T lymphocyte subgroup can assess changes of immune function in patients with lung cancer during perioperative period, postoperative lung cancer for clinical guidance in immunotherapy may provide a references for avoiding the corresponding complications caused by immune function declining, preventing lung cancer postoperative recurrence and metastasis and micro carcinoma metastases of rapid growth and a offering atheoretical reference during postoperative period.