Cytology And Molecular Epidemiological Study On Vit D Receptor Mediating The Effects Of Estrogen On Bone Metabolism

Objective: Postmenopausal osteoporosis is the level of estrogen sharply dropping which leads to the metabolic of bone imbalance after natural and physiology menopause for women.Long-term estrogen application may bring the risk of cardiovascular, breast cancer and so on. As the secure replacement of estrogen,we pay attention to phytoestrogens about prevention and treatment of postmenopausal osteoporosis. Studies have found that estrogen regulating bone metabolic may be mediated by Vit D receptor, but whether the bone metabolic regulation of phytoestrogen relating with VDR is unknown. We research whether phytoestrogen is involved in VDR in regulating bone metabolism from the perspective of cell and epidemiology.Methods:With different concentrations of Genistein(GEN) adding in MC3T3-E1 cells, using MTT to detect cell proliferation rate, we observe whether ZK159222 which is the blockers of VDR has an effect on GEN. After adding GEN in MC3T3 E1 cells, we use Western blotting to detect VDR protein expression. And after joining the estrogen receptor alpha blockers MPP, estrogen receptor beta blockers PHTPP,we determin if the role of GEN regulating the VDR protein expression can be canceled.Making epidemiological investigation about 300 cases of nanchang postmenopausal women blood samples, extracting DNA, we observe the every sample’s VDR gene Apa I and Bsm I restriction fragment length polymorphism, in combination with dietary survey and bone mineral density test results.We observe the correlation between dietary phytoestrogens and bone mineral densityin in different VDR gene polymorphism.Results: 10-8 M of MC3T3 E1- GEN can promote cell proliferation(p < 0.05), which can be canceled by VDR blocker ZK159222. 10-8 M GEN can increase the expression of VDR protein in MC3T3 E1 cell(p < 0.05) and not be canceled by MPP and PHTPP.Making an analysis on VDR restriction enzyme site Apa I and Bsm I genotype to find that the BMD of each part of different genotypes do not have significant difference(p > 0.05), and dietary phytoestrogen intake of different samples genotype is not associated with BMD(p > 0.05).Conclusion: 1. GEN can promote MC3T3 E1 cell proliferation through mediating VDR.2.GEN can increase the expression of VDR protein which is not mediated by estrogen receptor.3.VDR gene polymorphism has nothing to do with bone mineral density and bone metabolic effect of dietary phytoestrogen intake for postmenopausal women in Nan Chang.