| Pogostemonis Herba(PH) is a common used Traditional Chinese Medicinal herb.It is used to remove dampness, relieve summer-heat, stop vomiting and stimulate the appetite. Pericarrium Citri Retieultae(PCR) has the effect managing gas tonifying spleen and the eliminating dampness reducing phlegm. The compatibility of PH and PCR increased the efficacy of preventing vomiting, alleviate diarrhea and removing dampness, have been used to malaria of cholera, exogenous damp, or stuffy gastral cavity ruffian is full, eat less indigestion which caused by phlegm-damp.Presently, most attentions are paid to its pharmacological effects. For the purpose of making full use of the medicinal herb, several parts from herbal pair are studied.First, investigate the extraction condition of volatile oil distilled from herbal pair(HP) of Pogostemonis Herba(PH) and Pericarrium Citri Retieultae(PCR). Based on single factor experiment, with the theory of Response Surface Methodology,optimization of steam distillation of volatile oil extraction technology. The optimal conditions of extraction process were as follows: sift 60 mesh sieve, 10:1 for water:powder and 5h for extraction.Second, the volatile oil of the herbal pair, Pogostemonis Herba(PH) and Pericarrium Citri Retieultae(PCR) were analyzed by gas chromatography-mass spectrometry(GC-MS), with automated mass spectral deconvolution and identification system(AMDIS) based on the temperature-programmed retention in dex(PTRI). Comparing the difference between chromatographys to identify the main chemical constituents, and the relative concentration of each component was determined by peak area normalization.The results show that there are 85, 121 and134 kinds of constituents of the essential oils being identified respectively from single herb PCR, PH and PH-PCR, and the relative amount accounts for 78.79%, 82.80%and 84.01% of the total relative contents in order. There are 23 common volatile constitutions in single herb and PH-PCR, while there are 55 and 59 common volatile constitutions in the single herb PCR and PH respectively. The compatibility of the drug pair has an effect on components and contents in the volatile oil of PH and PCR.Their relative kinds and amount in PH-PCR are not equal to the two single herbs,whose reaction mechanism in PH-PCR should be studied further.Third, based on the theory of serum Pharmaceutical Chemistry, we established the analysis method for volatile oil from the herbal pair by using GC-MS. By the comparing the chromatogram of volatile oil from the herbal pair, blank SD rat’s plasma and dosed SD rat’s plasma, 20 original compounds from volatile oil and 2metabolites were identified.Last, to analyze the main component in volatile oil of PH-PCR with different compatibility proportions, and study on the content of the main compounds in volatile oil of PH-PCR compatibility of different compatibility proportions and try to explain the mechanism of this compatibility. In the found, the volume of main component,such as β-elemene, patchouli alcohol, display different degree of variations, some of them have been reported to have pharmacological effects.The chemical fingerprint in vitro and metabolic profile in vivo of herbal pair decoction were established in our study. Meanwhile, some chemical compounds especially flavonoids were isolated and identified by modern analysis techniques. A systemic method of isolation, purification, identification and screening of pharmacodynamic substance for chemical constituents in the TCM( traditional Chinese medicine)was established, which could supply a new thinking and scientific basis for the overall quality control of TCM. |
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