| This paper carried out the research of triherbal recipe (THR) FAI-CMO-RH comprehensively from herbal pairs point, which included FAI-RH, CMO-RH, FAI-CMO. The purpose of the paper is to clarify the relationship between the herb compatibility and the volatile chemical components. Chemical components of these essential oils were identified by gas chromatography-mass spectrometry (GC-MS) in herb fingerprint spectrum technology together with HELP and AMWFA methods of chemometric resolution method and temperature-programmed retention indices (PTRIs). Finally, the construction of volatile constituent spectrum in triherbal recipe (THR) was completed. In total, the paper mainly covers four parts as below:1. Construction and analysis of volatile constituent spectrum in herbal pair (HP) Fructus aurantii Immaturus (FAI)-Rhubarb (RH) and its single herbs. In total,64,19and33volatile chemical components in volatile oil of FAI, RH and HP FAI-RH were separately determined qualitatively. The main components of FAI included D-Limonene(66.06%),Benzene,1-methyl-4-(1-methylethyl)-(7.92%),1,4-Cyclohexadiene,1-methyl-4-(1-methylethyl)-(6.18%),1,6-Octadien-3-ol,3,7-dimethyl-(2.8%), n-Hexadecanoic acid (2.56%). The main components of RH included n-Hexadecanoic acid(56.85%),9,12-Octadecadienoic acid, methyl ester (14.91%), Cyclohexene,1-methyl-4-(1-methylethenyl)-,(S)-(6.94%),13-octadecenal,[Z]-(3.63%),9-Hexadecenoic acid(2.12%). And their herbal pair mainly included D-Limonene (33.02%), n-Hexadecanoic acid (25.63%),9,12-Octadecadienoic acid, methyl ester (7.54%), Benzene,1-methyl-4-(1-methylethyl)-(3.2%),1,4-Cyclohexadiene,1-methyl-4-(1-methyl ethyl)-(2.13%). There were21common volatile constituents in HP FAI-RH and single herb FAI,6common volatile constituents in HP FAI-RH and single herb RH and6common volatile constituents in FAI, RH and HP FAI-RH. Among the essential oils of HP FAI-RH,11new constituents appeared and55constituents disappeared. Through comparative analysis, the volatile constituents in HP were mainly derived from FAI, wether from the type or the content. 2. Construction and analysis of volatile constituent spectrum in herbal pair (HP) Cortex mangoliae Officinalis (CMO)-Rhubarb (RH) and its single herbs. The main components of CMO included (3-Eudesmol (26.57%), γ-Eudesmol (16.19%), n-Hexadecanoic acid (10.18%), α-Eudesmol (8.31%),9,12-Octadecadienoic acid, methyl ester (7.44%). And their herbal pair mainly included n-Hexadecanoic acid (30.10%),9,12-Octadecadienoic acid, methyl ester (23.62%), D-Limonene (8.77%), β-Eudesmol(7.82%), y-Eudesmol (7.8%). In total,26and45volatile chemical components in volatile oil of CMO and HP CMO-RH were separately determined qualitatively. There were17common volatile constituents in HP CMO-RH and single herb CMO,7common volatile constituents in HP CMO-RH and single herb RH and4common volatile constituents in CMO, RH and HP CMO-RH. Among the essential oils of HP CMO-RH,25new constituents appeared and20constituents disappeared. Generally speaking, the volatile constituents in HP were mainly derived from CMO. But the highest content volatile constituent in HP CMO-RH was from RH, Which represented30.10%of the total content of volatile oil in HP CMO-RH.3. Construction and analysis of volatile constituent spectrum in herbal pair (HP) Fructus aurantii Immaturus (FAI)-Cortex mangoliae Officinalis (CMO) and its single herbs. In total,49volatile constituents of HP FAI-CMO were checked out, which account for92.62%of the whole components. The main components of HP FAI-CMO included D-Limonene (32.21%), n-Hexadecanoic acid (17.7%),9,12-Octadecadienoic acid, methyl ester (9.12%), β-Eudesmol(6%), γ-Eudesmol(3.46%). There were36common volatile constituents in HP FAI-CMO and single herb FAI,10common volatile constituents in HP FAI-CMO and single herb CMO and7common volatile constituents in FAI, CMO and HP FAI-CMO. Among the essential oils of HP FAI-CMO,10new constituents appeared and41constituents disappeared. The volatile constituents in HP were derived from FAI, CMO in turn, followed by the content size.4. Construction and analysis of volatile constituent spectrum in triherbal recipe (THR) Fructus aurantii Immaturus (FAI)-Cortex mangoliae Officinalis (CMO)-Rhubarb (RH).41volatile constituents of THR FAI-CMO-RH were checked out, which represent94.31%of the whole components. The main components of THR FAI-CMO-RH included y-Eudesmol (15.6%), n-Hexadecanoic acid(14.45%), Eudesmol (10.6%), a-Eudesmol (10.1%), Propanoic acid,2-methoxy-,methyl ester(7.02%),9,12-Octadecadienoic acid (Z,Z)-(6.04%), β-Eudesmol (6.02%). There were14common volatile constituents in THR and HP FAI-RH,13common volatile constituents in THR and HP CMO-RH,16common volatile constituents in THR and HP FAI-CMO and11common volatile constituents in THR and its HPs. Among the essential oils of THR FAI-CMO-RH,9new constituents appeared and55constituents disappeared. Generally speaking, the volatile constituents in THR were mainly derived from HP FAI-CMO. Through comprehensive and systematic comparative analysis of chemical composition of THR and its HPs, this chapter explored the substance and law of the compound compatibility to clarify scientific connotation of the compatibility from the chemical composition point. Besides, the study also benefits the reseach on combination and demolition of recipe. |
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