The Study Of The Proliferation And Invasion Of BG-1 Ovarian Cancer Cell After Treated With Exogenous E₂

Objective To investigate the proliferation and invasion of BG-1 ovarian cancer cell line after treated with exogenous E2 and its underlying mechanism.Methods The impacts of exogenous E2 and various ER moderators on the proliferation of BG-1 ovarian cancer cell were measured by MTT assay. PCR and Western blot were used to analyze the expressions of cyclin D1 and p21 when BG-1 cells were treated with different drugs for 6 h, 24 h and 48 h. The expression of Matrix metalloproteinase-9(MMP-9) was evaluated by enzyme-linked immuno sorbent assay after treated with 10-9~10-5 mol/L E2 for 3 h, 6 h, 12 h and 24 h, respectively. The impacts of exogenous E2 and various ER moderators on the invasion of BG-1 ovarian cancer cell were detected by Transwell invasion assay.Results After the cells were treated with different concentrations of E2, the proliferations in different groups increased compared with blank control. Furthermore, the proliferat ive rate of cells in 10-7 mol/ L group was the highest among the others. The proliferation of E2+PPT group was significant ly higher than E2 group, while that of E2+ICI182,780 group was much lower than E2 group(P<0.05). There was no obvious alteration between E2+DPN group and E2 group(P>0.05). Compared with E2 group, cyclin D1 expressed much lower in E2+ICI182,780 group, but the expression of p21 was much higher than E2 group, the results in E2+PPT group were roughly opposite to E2+ICI182,780 group(P<0.05). There was no significant difference between E2+DPN group and E2 group(P>0.05). E2 dramatically increased the expression of MMP-9 at concentrations of 10-9-10-5 mol/L(P<0.05). Furthermore, the expression of MMP-9 in cells treated for 3 h with 10-8 mol/ L E2 was much higher than the others. In E2 group, the invasion was enhanc ed significantly in contrast with control group, while in E2+ICI182,780 group, it was weaken compared with control group, in E2+PPT group, it was enhanced(P<0.05).Conclusion E2 could promote the proliferation and invasion of BG-1 ovarian cancer cell line through ERα signal pathway. ICI182,780, one of ER blockers, has a significant antagonistic effect on it. ERα may become a new target of ovarian cancer gene therapy, which contributes to seek more reliable medicine in clinical.