Effect Of Trifluoperazine Combined Cyclophosphamide On Mesangial Cell Proliferation And Apoptosis In Chronic Anti Thy1 Glomerulonephritis Rat

Objective:explore the effect of trifluoperazine(TFP) combined cyclophosphamide(CTX) on mesangial cell proliferation and apoptosis in chronic resistant Thy1 glomerulonephritis rat, to clarify some of its protective effect and possible mechanism.Methods:40 male SD rats, and randomly selected 8 for normal control group, the remaining 32 preparation Thy1 glomerulonephritis model, The remaining 32 only preparation Thy1 glomerulonephritis model, 32 only resistant Thy1 glomerulonephritis rats were randomly divided into four groups Thy1 glomerulonephritis rats were randomly divided into four groups,each group of 8, respectively:(a) nephritis model group: don’t give any drug treatment;(b)TFP groups: intraperitoneal injection of TFP, 1 x per day, dose of 1.5 mg/kg;(c) CTX group:intraperitoneal injection of CTX once every 3 days, the dose of 150 mg/kg.(d) joint group:intraperitoneal injection of TFP, 1 x per day, dose of 1.5 mg/kg, intraperitoneal injection of CTX once every 3 days, the dose of 150 mg/kg.Groups began 0 d, after the start of the experiment in the experiment 2, 4, 6, 8 weekend put individual rats in metabolic cages 24 h urine specimen collection Testing 24 hours urinary protein, observed the pathological changes in kidney tissues, and semiquantitative analysis of glomerular damage degree,immunohistochemical detection of glomerular the expression of PCNA protein, western blot detection of Caspase- 3 protein expression.Results:(1) 2, 4, 6, 8 weekend 24 h urine protein quantity nephritis model group were significantly higher than that of normal control group(P < 0.05), treatment group 24 h urine protein quantity of rats were significantly lower than in 6, 8 weekend nephritis model group(P < 0.05), there was no statistically significant difference between the treatment group.Renal pathological changes(2) 8 weekend nephritis model group compared with blank control group glomerular damage serious(all P < 0.05), treatment group 3 pathological damage below nephritis model group(P < 0.05), especially the TFP + CTX group pathological damage the lightest, are lower than the other two groups(P < 0.05).(3) Immunohistochemical determination of PCNA expression in glomeruli,the expression of PCNA protein model control group compared with control group increased significantly(P < 0.05), 3 groups treatment can inhibit the expression of PCNA protein, notably the trifluoperazine combined cyclophosphamide group effect is remarkable, and the difference is statistically significant(P< 0.05).(4)Western blot method to detect the expression of Caspase-3 in the kidney tissues,the Caspase-3 protein expression of the model control group compared with normal control group decreased significantly(P < 0.05), three groups of treatment all can improve the Caspase- 3 protein expression, especially to trifluoperazine combined cyclophosphamide group effect is remarkable, and the difference is statistically significant(P < 0.05).Conclusions:cell cycle sequential administration by trifluoperazine combined cyclophosphamide can obviously improve the proliferative glomerulonephritis rats of pathological damage, can be effective to reduce the amount of urine protein proliferative glomerulonephritis rats,can reduce the expression of PCNA and inhibit mesangial cell proliferation,can increase the Caspase- 3protein expression and apoptosis induction of mesangial cells, in order to delay the progress of disease.