Effects Of IL-10 On The Proliferation And Differentiation Of Neural Stem Cells In Situ In The Rats After Cerebral Infarction

AbstractObject: It has been generally accepted that IL-10 have neuroprotection following cerebral infarction. In this study, we discussed the neuroprotection function and the mechanisms of IL-10 in rats with MCAO. Adult male SD rats were lateral ventricle injected IL-10 over-expression with IL-10 recombinanted lentiviral vector and IL-10 down-expression with recombinanted lentiviral vector-mediated IL-10 RNAi conditioning 1 hour after built MCAO model. Then the neurological severity were evaluated, the level of IL-10 were tested by using ELISA, Nestin and DCX were tested by using Western-Bolt in the ipsilateral brain.Methods:1. IL-10 over-expression recombinanted lentiviral vector(LV-IL-10) and IL-10 down-expression recombinanted lentiviral vector-mediated IL-10 RNAi(LV-IL10-RNAi) were established and packed.2. The MCAO model was set up by modified thread-occlusion method. The LV-IL-10(IL-10 over-expression) and LV-IL10-RNAi(IL-10 down-expression) were dissolved in PBS for lateral ventricle injection 1 hour after set up MCAO model. The expression of IL-10 were respectively measured in brain by using ELISA in each group on 7th day after MCAO. To understand the lentiviral transfection is successful or not.3.The NDS, modified neurological severity score(m NSS) and modified adhesive removal test were performed before and 7 days after MCAO to understand the changes of nerve function in each group rats. TTC staining and the percentage of infarct volumes were evaluated on 7th day after cerebral infarction to discuss the inhibition of apoptosis function of IL-10.4. The brains of rats taken out on 7th day after set up MCAO model. Protein expression level of Nestin, DCX were tested by Western-Bolt.Results: 1.ELISA result showed that the rats treated with IL-10 over-expressionrecombinanted lentiviral vector(LV-IL-10) can increased the level of IL-10, and treatedwith recombinanted lentiviral vector-mediated IL-10 RNAi(LV-IL10-RNAi) werecounter-productive. The results demonstrated that recombinanted lentiviral vector weretransfection successfully.2. The rats treated with IL-10 over-expression recombinanted lentiviral vector(LV-IL-10) can promote neurological deficit recovery following cerebral infarction andrestrain the percentage of brain infarct volumes. The rats treated with recombinantedlentiviral vector-mediated IL-10 RNAi(LV-IL10-RNAi) aggravated neurological deficitfollowing cerebral infarction and the percentage of brain infarct volumes.3.The rats treated with IL-10 over-expression recombinanted lentiviral vecto(LV-IL-10) can increased the level of Nestin and DCX protein. The rats treated withrecombinanted lentiviral vector-mediated IL-10 RNAi(LV-IL10-RNAi) can reduced thelevel of Nestin and DCX.Conclusion: IL-10 might have neuroprotection effect following cerebral infarction.The neuroprotection mechanism of IL-10 may be relate to the proliferation of NSC inrats following stroke.