Effect Of Different Fluid Resuscitation On Intestinal In Rats With Hemorrhagic Shock

Objective To investigate the morphology and immune inflammation of different fluid resuscitation on rats intestine in hemorrhagic shock.Methods A model of rat with hemorrhagic shock was established in 60 SD rats.The rats were randomly divided into Ringer resuscitation group(RL group), hypertonic saline(7.5%)resuscitation group(HTS group), hydroxyethyl starch resuscitation group(HES group),hypertonic saline(7.5%) combined with hydroxyethyl starch resuscitation group(HSH group).Each group contained 15 rats. MAP at different time points were recorded, the ultrastructure in intestine tissue was observed by electron microscope,the protein levels of NF-k B in peripheral blood monocytes were determined by enzyme-linked immunosorbent test,immunochemistry staining was used to detect the expression of ICAM-1 on intestinal mucosa and the apoptosis of intestinal mucosa was tested.Results At the time of fluid resuscitation is completed,compared with RL group 、 HES group、HSH group,the MAP of HTS group were observed(P<0.05). Pathological examination show the HSH group、HTS group were significantly lower than those in RL group、HES group(P<0.01).ICAM-1 and NF-k B detect show the HSH group 、 HTS group were significantly lower than those in RL group、HES group, HES group(P<0.05),but the levels of ICAM-1 and NF-k B expression in HSH group were significantly lower than those in HTS group(P<0.05).There was significant difference in apoptosis of intestinal mucosa in rats of RL、HES、HTS and HSH groups after hemorrhagic shock and resuscitation(P <0.05). Conclusion HSH can help protecting intestinal mucosa and suppress the expression of NF-k B、ICAM-1 and reduce apoptosis,which would be a better way of protecting the body cells and maintaining organ function.