Effect Of PDGF-D On Renal Fibroblasts In Renal Interstitial Fibrosis

OBJECTIVE:To investigate the effect of activation of PDGF-DD/Rβ signaling pathway on the proliferation 、 differentiation to Myo F and ECM expression of rat renal fibroblasts.METHODS:Cultured normal rat kidney interstitial fibroblast(NRK-49F) cells were divided into the following groups by PDGF-DD concentration:Control,1ng/ml,10ng/ml,50ng/ml,100ng/ml groups.And NRK-49F-s were then divided into four groups according to stimulation time of 50ng/ml PDGF-DD:Control、12h、24h、48h groups. After administrated by PDGF-DD,the Cell Counting Kit-8(CCK-8) assay was selected to detect the NRK-49 Fs proliferation. Reverse trancription polymerase chain reaction(RT-PCR) and Western blot were used to determine the m RNA and protein expression levels of PDGFR-β、α-SMA、collagen type Ⅰ、FN、MMP-2、TIMP-1 in all groups stimulated by different concentrations of PDGF-DD.RESULTS:PDGF-DD of different concentrations stimulated the proliferation of the NRK-49 Fs compared with that in control group(proliferation rates are 8.17%,19.17%,31.37%,30.77%,P<0.05);Proliferation rates of different stimulation time were higher than that in control group(12.70 %, 25.07 %, 39.79 %,P < 0.05).And PDGF-DD stimulated the PDGFR-β、α-SMA、collagen type Ⅰ、FN、TIMP-1 expression in m RNA and protein levels in an concentration dependent manner,but down regulated the expression of MMP-2,both m RNA and protein.CONCLUSION : PDGF-D/Rβ signaling pathway can obviously promote the proliferation of NRK-49 Fs,and fibroblasts in rats could be tranformed to myofibroblasts by PDGF-DD,PDGF-D also induce the deposition of extracellular matrix.