Correlation Studies Of MBL2,NLRP1 Genepoly Morphism With Autoimmune Thyroid Disease

Objective:Analyze gene polymorphism of the crowd in Shanxi Province suffering from autoimmune thyroid diseases of mannose binding lectin-2(MBL-2), as well as the combination of nucleotide-binding, leucine rich repeat pyrin domain containing protein 1(NLRP1), and explore its relationship with autoimmune thyroid disease susceptibility to provide a new idea for the pathogenesis of autoimmune thyroid and a new means for the treatment of autoimmune thyroid disease(AITD). Methods:PCR-RFLP were used to analyze the distribution of genotype in132 patients with AITD in Shanxi province(group GD, 62 cases; group HT, 70 cases) and 44 cases of normal control group MBL2-rs1800450A>G, NLRP1-rs12150220A>T and explore the relationship between it and susceptibility of GD and HT. Results:①The AA genotype dominated in MBL2-rs1800450A>G for pepole in Shanxi and allele is given priority to A. The frequency of AA genotype in control group were significantly higher than those in group GD(70.5% vs 35.5%) and was slightly higher than that of group HT(70.5% vs 54.3%). In group GD,there was statisically significant of MBL2-rs1800450A>G sites GG, AA, AG among three genotype compared with normal control group(P=0.002), while in group HT, there was no statistical significance compared with the control group(P=0.160).②The TA genotype dominated in NLRP1-rs12150220A>T with frequency of 50.0%, 55.7% and 45.5% for group GD, HT and the control group respectively. Most of alleles A were distributed in HT group and the control group and the frequency were 55.0%, 61.4% respectively, while allele T dominated in GD group and the frequency is 50.8%. Compared with control group three genotypes of AA, TA, TT in group GD, HT had no statistical difference(P=0.218, P=0.426).③The analysis of MBL2-rs1800450A>G polymorphism and the risk of GD, HT. Taking type AA for non-exposed factors and taking genotype AG, GG for risk factors, it was found in GD group, MBL2-rs1800450A>G, genotype AG OR=4.355(95%CI= 1.821-10.419), P<0.001 was statistically significant. The analysis of NLRP1-rs12150220 A>T polymorphism and the risk of GD, HT. Taking type AA for non-exposed factors and taking genotype TA, TT for risk factors, P>0.05, there was no statistically significant difference. Conclusions:①MBL2-rs1800450A>G loci gene polymorphism frequency distribution in Shanxi with AITD crowd was statistically significant compared with control group, individuals with AG genotype carrying the risk of suffering from GD are 4.355 times as much as that of the AA genotype crowd.②The distribution of NLRP1-rs12150220A>T gene polymorphism was not statistically significant among people in Shanxi and may not increase the risk of suffering from GD, HT.