The Roles And Mechanisms Of Mi R-203 Regulating ΔNP63 In Cervical Cancer Cells

Objective:Cervical cancer, a major gynecological cancer, which incidence is increasing and is great harmful to women’s health. Thus, to study the mechanism of cervical cancer, and to block the occurrence is still a problem to be solved. In recent years, studies have found that mi R-203 can regulate the expression of ΔNP63 in esophageal squamous cell and inhibit tumor growth and metastasis, which demonstrate that ΔNP63 may be one of the potential target genes for mi R-203. Futhermore, we confirmed that mi R-203 genes can combine with 3′UTR in the authoritive biological information website, which informed that mi R-203 may exert its biological functions by regulating the expression of ΔNp63.In conclusion,the purpose of this research is to explore the relationship of mi R-203 and ΔNP63 and verify whether mi R-203 can affect the biological behavior by targeting genes of ΔNP63 in cervical cancer cells ro not in order to eludicate the molecular mechanisms on cancer progression.Methods:1. Adopting gene prediction websit to clear the relationship of mi R-203 and Δ Np63.2. In Caski and C33 A, we use the cell transfection technology to overexpress or silence the mi R-203.3. mi R-203 was overexpressed and silenced in Caski and C33 A.CCK-8,flow cytometry, cell scratch test are conduced in all cell lines so as to observe the biological behaviore of cell proliferation, apoptosis and migration.4. RT-PCR is used to detect the quantity of mi R-203 and western blot is to ΔNp63protein for further study of potential mechanism of Mi R-203 in cervical cancer.Results:1. ΔNp63 is a potential target for mi R-203 predicted by bioinformatics target gene prediction website. MIR-203 can be highly combined with the m RNA 3’UTR of ΔNp63.2. HPV16-positive Caski cervical cancer cells and HPV16-negative C33 A cervical cancer cells were successfully transfected with mi R-203 mimics and mi R-203 inhibitor.3. The implications of the silence or overexpression of mi R-203 to biological characteristics in cancer cells: over-expression of mi R-203 may prevent cells proliferation, promote apoptosis, inhibit cell migration; and the silence of mi R-203 can enhance cell proliferation, delay apoptosis and promote cell migration.4. The relationship between mi R-203 and ΔNp63: the expression of mi R-203 were increased significantly in mimics transfected cells, ΔNp63 protein decreased obviously;whereas, mi R-203 was decreases in inhibitors transfected cells, ΔNp63 protein was increased.Conclusions:1. In cervical cancer cells, overexpression of mi R-203 may affect cells proliferation and apoptosis and migration, the silence of mi R-203 can accelerate cell proliferation,anti-apoptosis and promote cell migration. It suggested that mi R-203 can influence biological behaviour in Caski and C33 A.2. The overexpression of mi R-203 in cervical cancer cells, ΔNp63 protein were significantly reduced.On the contrary, the decreased mi R-203 in cervical cancer cells, the expression of ΔNp63 protein were significantly increased. mi R-203 may regulate ΔNp63for inhibit the development of cervical cancer.