The Infection And Partial Nucleotide Sequencing Of Hepatitis E Virus For People In Shihezi Of Xinjiang Province

Objective Xinjiang is the high incidence area of HE in Shihezi area. There are less detailed reports about HEV in the crowd prevalence, we hope that the passage of this experimental study of HEV in Shihezi in the crowd epidemiology and molecular epidemiology serum, the situation with a view to the future HEV further study and the prevention lay a good theoretical basis.Method By ELISA for detection of the anti-serum- HEV antibody, for some serum and stool specimens for HEV RNA detection, and positive PCR products were cloned and sequenced and partial gene sequence analysis.Result1. The overall positive rate of anti-HEV IgG was 26.10% in a total of 3513 people. While,in 1678 male and 1835 female, the positive rate was 25.98% and 26.21%.The antibody positive rate was 17.21%and 45.71% in urban population 2417 people and rural population of 1096 people.The positive rate of anti-HEV Ig G increased with age. Farmers and herdsmen antibodies infection rate that was 46.70% was highest,in different occupational groups of people.Different ethnic people have significant positive difference.2. This study in the serum of patients with acute HEV, feces detected three HEV RNA positive sample, sequence analysis showed that the strains between Changchun amino acid homology of 96.7% to100%,nucleotide homology are84.5% to 93.8%, select different genotypes ORF2 part of nucleotide and Shihezi three hepatitis E viruses(Sh-H-r,Sh-H-f-1,and Sh-H-f-2)conducted a comparative analysis, all of three Shihezi hepatitis E viruses are Type IV.Conclusion The results of HEV antibody positive rate was 26.10% in Shihezi, with higher infection rates.Shihezi three HEV gene are type IV.