The Research Of HIV Infection-related Gene Polymorphism And Prevalence Of Han, Yi And Hani Nationality In YUXI City Of YUNNAN Province

Background and ObjectTo investigate the distribution of geneloci CCR2b-64I, SDF1-3’A, CX3CR1-V249I and CX3CR1-T280M of Han, Yi and Hani Nationality in YUXI City of YUNNAN Province and the difference between Han, Yi, Hani nationality and other nationality with analysis the HIV infection-related gene mutation frequency and polymorphism, and comparison with results in domestic and abroad. Our research will provide theoretic basis for evaluate the HIV genetic susceptibility in Han, Yi, Hani nationality and HIV prophylaxis and treatment.Method1 The composition of HIV infected person registered in YUXI city of YUNNAN province since 1995 were analyzed. HIV infection situation were obtained with the analysis containing gender, career, marital status, route of infection and ethnic, and the diversification trends of these essential element mentioned before were analyzed with table and figure.2、Four geneloci CCR2b-64I, SDF1-3’A, CX3CR1-V249I and CX3CR1-T280M of Han, Yi and Hani nationality were detected and analyzed with SNP.The top 100-200 People from Han, Yi and Hani nationality were chosen according the ethnic composition in YUXI population and HIV infected person. Pure ethnic origin in three generation should be promised without consanguinity. The DNA were collected, and Four genelocis CCR2b-64I, SDF1-3’A, CX3CR1-V249I and CX3CR1-T280M were detected with MassArray and analyzed with SNP, then group representation of selected sample were detected with Hardy-Weinberg balance.3 Mutation frequency and polymorphism features of HIV infection-related gene of Han, Yi and Hani nationality were compared and analyzed with research results in domestic and abroad.To figure out the Mutation frequency and polymorphism features of HIV infection-related gene of Han, Yi and Hani nationality in YUXI City of YUNNAN province though studies mentioned above, and compared with research results in domestic and abroad, and the difference of these four genelocis distribution between Han, Yi and Hani nationality in YUXI City and other nationalities.Result1 Analysis of HIV infection in YUXI 1995 to 20111.1 The gender construction and diversification trendMale formed the majority in HIV infected group, but proportion of female were gradually increased in early few years, and became stabilized after 2007,the proportion were stabled at 40%.1.2 The occupation composition and diversification trend of infected personWorker, farmer, long-distance driver and commercial waiter formed the majority part in early years, and gradually spread to normal person, cause very extensively distribution in occupation since 2005, even spread to the low-risk population concluding children, students, teather and retirees, showing younger-aged character, and also the trace of infection in family.1.3 The marriage composition and diversification trend of infected personThe proportion of married infected person were gradually increased year by year to 50% at 2005, and became stabilized after 2007.1.4 The route of infection and diversification trend of infected personThe route of infection were drug injection in early years, but gradually conversed to sexual encounter, which has became the major infection way since 2006 with heterosexual contact form the majority, and homosexual contact apparently increased since 2006.1.5 The ethnic composition and diversification trend of infected personThe proportion of Han nationality of infected person was straightly decreased, and the proportion of minority gradually increased to nearly 50% until 2011. The number of ethnic of infected person were expanded to 16 ethnic from single Han nationality, and Yi, Hani, Dai and Hui nationality from the majority in this group. The infection proportion of minority (33.9%) concluding Hani, Dai, Hui without Yi nationality has already beyond their demographic ratio (31.69%).2 The SNP result of four geneloci2.1 The examination for group representationThe distribution of four geneloci of all subject were examined with X2 test, which in accordance with Hardy-Weinbery balance (p> 0.05), to indicated that all subject came from same group with good representativeness.2.2 The genotypic and allclomorphic gene frequency of four geneloci in different group205 samples were collected from Han nationality, and there were 168 samples for SDF1-3’A,169 samples for CCR2-64I,169 samples for CX3CR1-V249I and 169 samples for CX3CR1-T280M in successfully genetype sample of four geneloci; 100 samples were collected from Yi nationality, and there were 96 samples for SDF1-3’A,97 samples for CCR2-64I,96 samples for CX3CR1-V249I and 96 samples for CX3CR1-T280M in successfully genetype sample of four geneloci; 105 samples were collected from Hani nationality, and there were 75 samples for SDF1-3’A,78 samples for CCR2-64I,77 samples for CX3CR1-V249I and 77 samples for CX3CR1-T280M in successfully genetype sample of four geneloci. There were statistically difference of SDF1-3’A genotypic frequency (p=0.024< 0.05) and allclomorphic gene frequency (p=0.008< 0.01) in Han and Yi nationality; there were statistically difference of CCR2-64I genotypic frequency (p=0.008< 0.01) in Han and Hani nationality; there were no statistically difference of CX3CR1-V249I and CX3CR1-T280M genotypic frequency and allclomorphic gene frequency in Han and Hani nationality;2 The linkage between 2491 and 280M of CX3CR1CX3CR1 were detected with SNP in 342 samples from these three nationalities, the linkage disequilibrium coefficient between allclomorphic gene 2491 and 280M of CX3CR1 was 0.962, which show apparently linkage.2.4 The comparison between the four geneloci and obtained result in domestic2.4.1 The comparison between the result of SDF1-3’A geneloci typing and obtained result in domestic and abroadTo compared with obtained result in domestic and abroad by mutation frequency which present with A allclomorphic gene. There was no statistically difference of obtained result in mutation of Han nationality between in this research and in domestic research; obtained result of mutation frequency of Han was apparently higher than nationalities Oroqun (p=0.024), Ewenki (p=0.024) and Drung (p=0.018), lower than Molao nationality (p=0.025), which was statistically difference between these nationalities mentioned above; the mutation frequency of Han was apparently higher than negro in America (p=0.000) with statistically difference but propinquity with xanthoderm in Asia, Caucasus and Spanish when compared with obtained result of abroad. There were no research about this geneloci in domestic, there was no statistically difference in the mutation frequency result obtained in this research between Yi and Han nationality, except in North China, the mutation frequency was higher of Yi nationality than Han nationality (p=0.028); when compared with minority, the mutation frequency of Yi nationality was apparently higher than nationalities Oroqun (p=0.001), Ewenki (p=0.001), Drung (p=0.001), Wa (Cayuan county) (p=0.004), Lisu (Fugong county) (p=0.004), Nu (Fugong county) (p=0.008), Nu (Gongshan county) (p=0.002), Jingpo (Dehong county) (p=0.007), She (p=0.008), with statistically difference; the mutation frequency of Yi was apparently higher than negro in America (p=0.000), Caucasus (p=0.003) and Spanish (p=0.002) with statistically difference but propinquity with xanthoderm in Asia when compared with obtained result of abroad. There was no statistically difference in the mutation frequency result obtained in this research between Hani and Han nationality; when compared with minority, the mutation frequency of Yi nationality was apparently higher than nationalities Oroqun (p=0.018), Ewenki (p=0.018), Drung (p=0.013), Nu (Gongshan county) (p=0.037) with statistically difference; there was some difference were compared with obtained result of other ethnic in domestic, but no statistically difference; the mutation frequency of Hani was apparently higher than negro in America (p=0.000) with statistically difference but propinquity with xanthoderm in Asia, Caucasus and Spanish when compared with obtained result of abroad. The mutation frequency of Yi nationality was highest in three nationalities in this research, apparently higher than other two nationalities. The difference were maximum when compared with Han nationality, but no statistically difference (p=0.057).2.4.2 The comparison between the result of CCR2-64I geneloci typing and obtained result in domestic and abroadTo compared with obtained result in domestic and abroad by mutation frequency which present with A allclomorphic gene. There was no statistically difference of obtained result in mutation of Han nationality between in this research and in domestic research; obtained result of mutation frequency of Han was apparently higher than minority nationalities Tajik (p=0.042), Nu (Gongshan county)(p=0.020), Pumi(p=0.015), Yi (Liangshan, Sichuang province) (p=0.014), Buyi (p=0.044), Li (p=0.000), Molao (Guangxi province) (p=0.001) with statistically difference; the mutation frequency of Han was apparently higher than negro in America (p=0.003) and Caucasus (p=0.000) with statistically difference when compared with obtained result of abroad. The mutation frequency of Yi nationality in this research was apparently higher than Liang Shan, Sichuang province with statistically difference; the mutation frequency of Yi nationality was apparently higher than nationalities Tu (p=0.014), Tajik (p=0.008), Han (Gansu province) (p=0.046), Han (capital Beijing) (p=0.006), Uighur (Kashmir) (p=0.019), Drung (p=0.042), Wa (Cangyuan county) (p=0.044), Nu (Gongshan county) (p=0.003), Bai (p=0.010), Dai (p=0.011), Pumi (p=0.002), Jingpo (Dehong county) (p=0.014),Buyi (p=0.006),Li (p=0.000), Molao (Guangxi province) (p=0.000),Zhuang (Guangxi province) (p=0.000) with statistically difference; the mutation frequency of Yi was apparently higher than negro in America (p=0.000) and Caucasus (p=0.000) with statistically difference when compared with obtained result of abroad. There was no statistically difference of the mutation frequency of Hani nationality in this research and other domestic Hani branch; the mutation frequency of Hani nationality was apparently higher than nationalities Tu (p=0.007), Tajik (p=0.004), Han (Gansu province) (p=0.023), Han (capital Beijing) (p=0.001), Uighur (Kashmir) (p=0.005), Drung(p=0.019), Wa (Canyuan county) (p=0.020), Wa (Ximeng county) (p=0.028),Nu(Fugong county) (p=0.046),,Nu (Gongshan county) (p=0.001),Bai (p=0.004),Dai (p=0.004), Pumi (p=0.001), Jingpo (Dehong county) (p=0.005), Yi (Liangshan, Sichuang province) (p=0.000),Buyi (p=0.002),Li (p=0.000),Molao(Guangxi province) (p=0.000), Zhuang (Guangxi province) (p=0.000) with statistically difference; the mutation frequency of Hani was apparently higher than negro in America (p=0.000) and Caucasus (p=0.000) with statistically difference and higher than Spanish (there was no statistically analysis because lack samples) when compared with obtained result of abroad. The mutation frequency of Hani nationality was highest in three nationalities in this research, apparently higher than other two nationalities. The difference were maximum when compared with Han nationality, but no statistically difference (p=0.103).2.4.3 The comparison between the result of CX3CR1 geneloci typing and obtained result in domestic and abroadTo compared with obtained result in domestic and abroad by mutation frequency which present with A and T allclomorphic gene. The allclomorphic gene frequency of CX3CR1 was general low, even approach zero in Li and Zhuang nationality and below 0.1 in other nationalities. There was no statistically difference of obtained result in mutation of Han nationality between in this research and in domestic research; there was no statistically difference in Han nationality in this research when compared with in domestic and abroad, except apparently lower than nationality Lisu (Liuku county) (p=0.001), Tajik (p=0.009), Uighur (Xinjiang province) (p=0.009),French (p=0.000), Italian (p=0.000), Caucasus (p=0.000) at V249I locus with statistically difference, and apparently lower than nationality Lisu (Liuku county) (p=0.001), Tajik (p=0.005), Uighur (Xinjiang province) (p=0.005),French (p=0.000),Italian(p=0.000), Caucasus (p=0.000) at T280M locus with statistically difference. There was no statistically difference in other nationalities in this research when compared with in domestic and abroad; There was no statistically difference of obtained result in mutation of Yi nationality between in this research and in domestic research; but apparently lower than nationality Lisu (Liuku county) (p=0.005), Tajik (p=0.021), Uighur (Xinjiang provice) (p=0.026),French (p=0.000),Italian (p=0.000) and Caucasus (p=0.000) at V249I locus with statistically difference, there was no statistically difference in other nationalities in this research when compared with in domestic and abroad; and apparently lower than nationality Lisu (Liuku county) (p=0.024),French (p=0.049), Italian (p=0.034), Caucasus (p=0.038) at T280M locus with statistically difference, there was no statistically difference in other nationalities in this research when compared with in domestic. There was no statistically difference of obtained result in mutation of Yi nationality between in this research and in domestic research; but apparently lower than nationality Lisu (Liuku county) (p=0.004), Tajik (p=0.013), Uighur (Xinjiang province) (p=0.016).French (p=0.000), Italian (p=0.000) and Caucasus (p=0.000) at V249I locus with statistically difference, there was no statistically difference in other nationalities in this research when compared with in domestic and abroad; and also apparently lower than nationality Lisu (Liuku county) (p=0.001),French (p=0.003), Italian (p=0.002), Caucasus (p=0.003) at T280M locus with statistically difference, there was no statistically difference in other nationalities in this research when compared with in domestic and abroad. The mutation frequency difference was low in these three nationalities.ConclusionThe CCR2b-64I, SDF1-3’A, CX3CR1-V249I and CX3CR1-T280M geneloci in Han, Yi and Hani nationality of Yunnan province were studied detailed with SNP. The conclusion can be obtained with the SNP synthesize analysis of the HIV infected prevalence in Yuxi, Yunnan province and the CCR2b-64I, SDF1-3’A, CX3CR1-V249I, CX3CR1-T280M in different nationalities in domestic and abroad:1 The prevalence in Yuxi behaved characters below:(1) Male formed the majority in the infected group, but the major route of infection was heterosexual contact, which indicated that the key point is to supervision with HIV propaganda and intermeddle for infected female, especially for prostitutes; (2) The proportion of infected married person were gradually increased, indicated that the infected problem in family became outstanding; (3) HIV has already spread to the Low-risk group as students, teachers and retirees; (4) The rural population with lower education degree formed the majority of the infected group, minority has been already became a major infected group with rapidly increased speed.2 There was general character in result of the three nationalities in this research and other nationalities in domestic, but notable different character of each of them were exist, such as apparently linkage between 2491 and 280M even though with the same low allclomorphic gene frequency of CX3CR1, which was the major morbigenous factor; the high allclomorphic gene frequency of CCR2b-64I and SDFl-3’which were the major guardian factors, especially in Yi nationality; there were apparently difference in these three nationalities with different characters; the mutation frequency of CCR2-64I in Yi nationality in this research was apparently higher than Yi nationality in liangshan, Sichuang province of china, which indicated the huge influence from environment.3 The CCR2b-64I, SDF1-3’A and CX3CR1 were the hot topic in today’s research, and they also be mature allclomorphic geneloci. Considering the complete study result of these three geneloci was still the shortage in domestic, this research fulfilled this shortage in domestic, and abounded and completed the genetic background data of HIV susceptibility and antivenereal in Chinese minority.