Expression Of WNT5B In Breast Cancer And Clinical Significance

AIM:To study the expression of WNT5B in the breast cancer and further to discuss the correlation between WNT5B and clinical-pathologic characteristics of breast cancer.METHODS:The expression of WNT5B at both mRNA and protein levels was measured respectively by real-time fluorescent quantitative PCR and Western blotting in 67 cases of breast cancer and normal tissue adjacent to carcinoma. In addition, the immunohistochemical (IHC) method was used to detect the expression of WNT5B in breast cancer and normal tissue adjacent to carcinoma. The relationship between WNT5B expression and clinical pathologic indexes was also analyzed.RESULTS:The expression of WNT5B in the breast cancer was obviously lower than in normal tissue adjacent to carcinoma (P<0.05), There expression of WNT5B at mRNA and protein levels in 67 samples of breast cancer was in various degrees. The expression of WNT5B in the group T<20 mm of human breast cancer was obviously higher than that in the group T>20 mm (P<0.05). The expression of WNT5B had no obvious correlation with axillary lymph node metastasis, histological grade and immunohistochemical indexes of C-erBb-2, ER, ER, P53 and Ki67 (P>0.05) in the breast cancer.CONCLUSION:The expression of WNT5B decreases obviously in breast cancer. The expression of WNT5B is related to primary tumor size, which provides new ideas for the diagnosis and treatment of breast cancer, suggesting that WNT5B may be a new molecular marker for poor prognosis of breast cancer.