To Explore The Effects Of BMSCs Transplantation Combined With Chabc Degradation On Recovery Of Function After Spinal Cord Injury

Objective:To explore the effects of BMSCs transplantation combined with chondroitinase ABC degradation on the repair of nerve function after spinal cord injury in rats.Methods:60 adult male SD rats were used to established the spinal cord injury model and were divided into four groups randomly (15 rats/group), including Spinal cord injury control group(shown as group A), BMSCs transplantation group (shown as group B), ChABC injection group (shown as group C), and BMSCs combined with ChABC injection transplantation group (shown as group D). The movement function of rats was observed before they were killed at 7 and 14 days after injury, respectively. At 7 and 14 days after injury, BBB score method was used to evaluation the rat hind legs feeling of movement function;At 7 days after injury, the apoptotic level of neuronal cell was deleted using TUNEL assays. At 14 days after injury, The total area of the damage zone was evaluated using HE staining; Moreover the expression of GFAP and NF200/Nogo-A in injured adult rat spinal cord was detected through immunohistochemical technique.Results:During 7 days after injury, no significant differences of the BBB score were found among 4 groups (P=0.892); At 14 days after injury, Four group of BBB scores were as follows:(6.33±1.000), (7.25±0.957), (7.33± 0.577) and (9.75±0.957). respectively. The BBB score of group D was obviously higher than that of the other 3 groups (P<0.05);14 days after the injury, The relative void area of the cavities formed in the damage zone were as follows:(4.44±0.18), (3.70±0.21), (3.23±0.22), (2.33± 0.31), respectively. Among these, the damage area of group D was significantly smaller than that of the other 3 groups (P<0.05); Moreover, that the expression of GFAP/Nogo-A in group D was obviously decreased than that of the other 3 groups (p<0.05),while the expression of NF200 in group D was obviously higher than that of the other 3 groups (P<0.05) at 14 days after the injury. Lastly, TUNEL assays showed that the apoptosis rate of cells in group D was significantly lower than the other three groups at 7 days after injury (P<0.05).Conclusion:BMSCs transplantation combined ChABC degradation could dissolve glial scar, reduce neuronal apoptosis, and promote the regeneration of nerve fibers and cross regional spinal cord injury, suggesting significantly repair effect after spinal cord injury.