Early Hyperbaric Oxygen Combined MP Treatment On Spinal Cord Injury Rabbit With Seawater Immersion

Objective To establish a model about spinal cord injury of rabbit with seawater immersion, observe the affection of HBO groups, MP groups and the Combined intervention groups about spinal cord injury of the model, evaluate and compare the therapeutic effect of three methods.Methods Eighty rabbits were randomly divided into 4 groups, the weight of rabbits among 2.5 to 3 kg and ignore the male and female. Each group contains 20. The rabbit model of 10 th thoracic spinal cord injury was reproduced by the modified Allen’s method followed by seawater immersion. Respectively given Blank control, HPO, MP and combination group after the models were accomplished. Rabbits were randomly selected from each group at 6, 12, 24 and 48 hours after immersion, and the neurological function of rabbits were evaluated by using the Tarlov scale, then the animals were sacrificed, and the histopathological features were studied with light microscopy, the expressions of Bax.Bcl-2 and NF200 were determined by immunohistochemistry, and the apoptosis of neurons in spinal cord was evaluated by TUNEL method.Results Both of the Tarlov scale and Histopathological index in The HBP group or MP group are significantly superior to Blank control,the differences were significant(P<0.05),The index of correlation in combination treatment group are superior to the other treatment,group the differences were significant(P<0.05).Conclusion The separate application of HPO or MP can accelerate the spinal cord injury healing and protect the neurons of spinal cord injury with seawater immersion by reducing the expression of Bax and promote the expression of Bcl-2 and NF200. In addition, the combination of two methods can improve the therapeutic effect.