The Clinical Analysis Of Peripheral Blood Lymphocyte Immunophenotyping In Interstitial Lung Lesions

Objective Diffuse interstitial lung disease(DILD) is a set of clinical features are similar,different immune pathological process of pulmonary disease of disease,unknown etiology is 65%, its pathogenesis is not yet fully understood. Viruses,bacteria, fungi, inhaling harmful gas, drug can result in interstitial lung disease,rheumatism, etc. ILD covers many diseases, each has its clinical characteristics, diagnosis has its difficulties, treatment and prognosis for diagnosis. In the diagnosis and differential diagnosis of interstitial lung disease of connective tissue disease occupies an important position. Connective tissue disease appeared interstitial lung lesions, is difficult to judge infection, or abnormal immune cause lung injury, it is difficult to identify the clinical situation.If can through laboratory index accurate judgment interstitial lung disease is caused by infection, or immune injury caused by, or both, to guide clinical treatment, focusing on the anti-infection treatment, or application of hormones or immunosuppressive therapy, or anti-infection and immunosuppressant therapy and has important significance. Now based on the detection of connective tissue diseases, connective tissue disease and normal peripheral blood lymphocytes of the immune classification changes, understanding of T cells, B cells and NK cells in the connective tissue disease patients complicated with pulmonary interstitial lesions, provide the certain reference value for guiding clinical treatment.Purpose Explore the peripheral blood lymphocyte immune classification and NK cell level change for identifying the cause of connective tissue disease complicated by pulmonary interstitial lesions of clinical value.Methods This topic selection of in China-Japan Friendship Hospital, Jilin University from May 2012 to December 2014 admitted during the period of 60 patients with interstitial lung lesions. Among 30 cases of connective tissue disease patients complicated with interstitial lung disease, diagnosis according to the 2012 version of a full set of guidelines for standard rheumatism, into the connective tissue disease group; 30 cases of connective tissue disease patients complicated with interstitial lung disease, into a group of connective tissue disease; 25 cases of healthy people as the normal control group; Using flow cytometry detection of 30 patients with connective tissue disease group, 30 cases of patients with connective tissue disease group, 25 cases of healthy people in the peripheral blood of CD3 +, CD4 +, CD8 + CD56 + NK cells, CD19+ B lymphocytes expression level. And the general clinical data of two groups of patients, the main clinical symptoms, chest CT image change, comparing the incidence of respiratory failure.Results Connective tissue disease group compared with the connective tissue disease group, CD8 + T cells were significantly higher(p < 0.01); Connective tissue disease group compared with normal control group, CD8 + T cells also increased obviously, but the number of CD56 + significantly reduced, all p <0.05; The connective tissue disease group compared with normal control group, the peripheral blood T cells subgroup had no obvious difference.Connective tissue disease group and the connective tissue disease, compared to the general clinical data of two groups of patients, the main clinical symptoms, chest CT image change, the incidence of respiratory failure were no significant difference(p > 0.05).Discussions Peripheral blood CD8 + T cells, connective tissue disease group was obviously increased, the number of NK cells significantly decreased; The connective tissue disease group of peripheral blood T cells each subgroup not seen obvious abnormity, tip peripheral blood lymphocyte immune classification helps to identify caused by damage to the immunity of the connective tissue disease complicated with interstitial lung disease.Clinical manifestations, chest CT image change, whether the occurrence of respiratory failure for connective tissue disease complicated by pulmonary interstitial disease and other causes of pulmonary interstitial change without value in differential diagnosis.