Detection Of CD4+CD25+ Regulatory T Cell In Patients With Graves’ Ophthalmopathy

Objective:1.To observe the change of autoimmunity in the patients with Graves’ ophthalmopathy(GO) in the way of detecting the ratio of CD4+CD25+ regulatory T cell in blood. 2.To probe into new detection index of the development and progression of Graves’ ophthalmopathy(GO),and to provide a new clue for prevention and treatment.Methods:1.Whole blood samples were obtained from 53 GO patients without exophthalmos,51 GO patients with exophthalmos, and 51 healthy people.2.Mononuclear cells were extracted in each sample respectively.3.The cells were labeled by Monoclonal antibodies (CD4-PC5、CD25-FITC and FoxP3-PE).4.The expression rates of the target cells were screened and measured by flow cytometry (FCM).Results:1.Data were analyzed by Kruskal-Wallis rank sum test.When there were differences between groups, data were analyzed by Bonferroni test between groups again.2. In GO patients without exophthalmos group,the ratio of CD4+CD25+T cells was lower than that in healthy controller group (P<0.01). In GO patients with exophthalmos group,the ratio of CD4+CD25+T cells was far lower than that in healthy c ontroller group (P<0.01). Compared with the ratio of GO patients without exophthalmos group, that of GO patients with exophthalmos group was also much lower(P<0.01).Conclusion:The ratio of CD4+CD25+T cell in the patients of GO group get low.Thin king of the autoimmune disorder in this disease, perhaps the ratio of CD4+CD25+T cell reducing is closely related to the patient’s autoimmune disorder, that provides a new direc tion for immunological research.