The Investigation Of The Aqueous Extract Of Ephedra Sinica Treating In Brain Damage After Subarachnoid Hemorrhage In Rats

Objective:To observe the therapeutic effect and mechanism of the aqueous extract of ephedra sinica on brain damage after subarachnoid hemorrhage (SAH) in rats.Methods:50 rats of Sprague-Dawley were randomly divided into control group, model group and three groups treated with different concentrations (4mg/kg,12mg/kg,36 mg/kg).The changes of the cerebral water content, malondialdehyde (MDA), glutathione peroxidase (GSH-Px) and hydroxy radical of brain tissue were analyzed, and hematoxylin-eosin (HE) staining was used to test the subarachnoid haemorrhagia and oedema, and immunohistochemistry and western blot were carried out to assay the expression of complement C3 in brains of different animal in different group 3d after operation.Results:On the postoperative 3days,compared with the model group,the content of MDA、GSH-Px activity and hydroxyl radical of 12、 36mg/kg treatment groups significantly reduced(P<0.01),the content of MDA of 4mg/kg treatment group not change(P>0.05),but GSH-Px activity and inhibition of hydroxyl radical significantly reduced(P<0.01),and the cerebral water content of 12、36mg/kg groups were obviously lower compared with model group. Through western blot and immunohistochemistry showed the expression of complement C3 was significantly lower on 36mg/kg treatment group and edema reduced.Conclusion The aqueous extract of ephedra could significantly inhibit the activity of complement C3,prevent the production of MDA、GSH-Px and hydroxyl radical, reduce the severity of cerebral edema and the inflammatory response, which has a better therapeutic effect SAH animals.