Research On Syndrome Of Dampness In Middle Jiao Model Rats Affecting Water Contransports Protein Of Solute Carrier 12 Family By Pingwei Powder

Objective:To explore the affect on water contransports protein of solute carrier 12 family under Syndrome of dampness in middle Jiao animal model and the relationship by Pingwei Powder intervention.Thus reveals the mechanisms of water metabolism disorders under Syndrome of dampness in middle Jiao and the mechanisms of water metabolism regulation by Pingwei Powder.Method:Using pre-mature methods establish a stable animal model of dampness Then detecting Na+-K+-ATPase activity by protein quantification,measured D-xylose content by phloroglucinol method,and measured KCC1, KCC3, KCC4, NKCC1 content in lung, liver and kidney tissue by enzyme-linked immunosorbent assay competitive method.Results:1.Successfully established model of dampness in the middle jiao① There are some significant change in dampness model group compared with the control group model by macro-indicators:poor mental state,eating less,obviously weight los. ② there are some changs in dampness model such as delayed gastric emptying, lower serum D-xylose absorption and inhibit Na+-K+-ATPase enzyme activity by objective indicators detection. ③ After interventioned by Pingwei powder,diet, weight, gastric emptying, D-xylose absorption and Na+-K+-ATPase enzyme are make better. Dampness can speculate successful model in rats.While it could be conclusion that the dampness model in rats modeling successfully.2 Na+-K+-ATP activity in liver, lung and kidney2.1 Na+-K+-ATP activity of dampness in the middle jiao modelCompared with the control group, the Na+-K+-ATP activity of lung,liver and kidney in dampness model are decreased;then the natural recovery group decline further,while kidney Na+-K+-ATP activity returned to normal levels.2.2 The impact of drugs Interventionon the Na+-K+-ATP activity activityCompared with the natural recovery group,Na+-K+-ATP activity of low-dose group increased significantly in lung and liver,while in kidney just present declining trend;while Na+-K+-ATP activity of positive group in lung and liver are increased,but significantly decreased in kidney.3 Water cotransporter content3.1 Express increased of water cotransporter contentCompared with the control group.the content of KCC1 in kidney,KCC3 and NKCC1 in lung and liver, KCC4 in kidney with model group;recovery group of KCC3, NKCC1 in lungs, KCC1, KCC3,NKCC1 in liver, KCC4 in kidney are increased.3.2Express decreased of water cotransporter contentCompared with the control group,the content of KCC1 in lung,KCC3 and NKCC1 in kidney with modelgroup;recovery group of KCCland KCC4 in lung,KCC4 in liver, KCC1、KCC3、NKCC1 in kidney are decreased.4 water cotransporter content after drugs intervent4.1 Water cotransporter content increased after drugs interventCompared with the model group, the intervention group of KCC1, KCC3, KCC4, NKCC1 in lung,KCC3,KCC4 and NKCC1 in liver, KCC3, KCC4 in kidney are increased. Furosemide group of KCC3 in lung,KCC1 in liver, Pingwei Gase diarrhea group of KCC4 in lungs, KCC1 in liver are increased.4.2Water cotransporter content decreased after drugs interventCompared with the model group,the intervention group content of KCC1,KCC3,KCC4 and NKCC1 in lung,KCC1 in liver,NKCC1 in kidney are decreased. Furosemide group content of KCC1, KCC4, NKCC1 in lung,liver KCC1,KCC1, KCC3, KCC4 and NKCC1 in kidney Pingwei Gase diarrhea group lungs KCC4, liver KCC1, KCC4, KCC1, KCC3, KCC4, NKCC1 in kidney are decreased.Conclusion:Syndrome of dampness in middle Jiao model not only can affect energy metabolism, but also can affect KCC1, KCC3, KCC4, NKCC1 levels of expression in the liver, lung and kidney, it may be one of the mechanisms caused the syndrome of dampness in middle Jiao that water metabolism disorders; Pingwei can regulation Na+-K+-ATPase activity and KCC1, KCC3, KCC4, NKCC1 content distribution in liver, lung and kidney,that also involved in energy metabolism and regulation water metabolism, which may be one of the mechanisms to improve dampness by pingwei powder.