Effects Of Astragalus Polysaccharide On The Invasive Ability Of Cervical Carcinoma C-4I Cells

Background and ObjectivesCervical carcinoma is a common malignant tumor in women, the incidence is second only to breast carcinoma. Invasion is the key factors affecting quality of life and prognosis of patients with cervical carcinoma. So, exploration of the mechanism and inhibitive method of the invasion of cervical carcinoma have important significance. The anti-tumor effect of traditional Chinese medicine has attracted more and more attention. Astragalus polysaccharide (APS) is the important active component of astragalus. Many studies had showed that APS had anti-tumor activity. However, the mechanism of APS anti-tumor is not yet fully understood. so far, there is no report about the effects of APS on the invasive ability of cervical carcinoma C-4I cells. The aims of this study are to study the effects of APS on invasive ability of cervical carcinoma C-4I cells and its molecular mechanisms, and provide scientific evidence for the feasibility of APS application in cervical carcinoma therapy.Methods1. APS was extracted and quantitated.2. Based on the results of previous studies, the experimental group was set to cervical carcinoma C-4I cells treated with 200μg/mL APS for 48h, while the control group was set to cervical carcinoma C-4I cells untreated with APS.3. The effects of APS on migrative and invasive ability of cervical carcinoma C-4I cells were discussed via scarification assay, transwell migration and cell invasion assay.4. The changes of E-cadherin, matrix metallo-proteinase-9 (MMP-9) and MMP-2 in cervical carcinoma C-4I cells treated and untreated with APS were tested by Western blotting assay.Results1. The concentration of prepared APS solution was 61.56mg/mL.According to the results of effects of Astragalus Polysaccharide on the Growth of Cervical Carcinoma C-4I Cells, in this study,the experimental group was set to cervical carcinoma C-4I cells treated with 200μg/mL APS for 48h, while the control group was set to cervical carcinoma C-4I cells untreated with APS.2. The results of scarification test revealed that the speed of wound healing of cervical carcinoma C-4Icells treated with 200μg/mL APS for 48h in experimental group was slower than the cervical carcinoma C-4I cells untreated with APS in control group. The wounds in control group C-4I cells 24h wound healing of 1/2,48h than two-thirds, and the complete healing was 72h, when the C-4I cells wound could be still observed at 72h in experimental group.3. The results of transwell migration and cell invasion test indicated that, compared with the number of cervical carcinoma C-4I cells untreated with APS in control group through basement membrane of chamber of control group, the number of cervical carcinoma C-4I cells treated with 200μg/mL APS for 48h in experimental group through basement membrane of chamber was strikingly decreased (P<0.01).4.The results of Western blotting assay showed that, the expressions of MMP-2 and MMP-9 were no significant difference between cervical carcinoma C-4I cells of control grou p and experimental group (P>0.05). But, compared with cervical carcinoma C-4I cells of control group, the active type of MMP-2 was reduced significantly in cervical carc inoma C-4I cells of experimental group (P<0.01), the active bands of MMP-9 were no t found in cervical carcinoma C-4I cells of control group and experimental group. The expression of E-cadherin in cervical carcinoma C-4I cells of experimental group was significantly increased than that in cervical carcinoma C-4I cells of control group (P< 0.01).ConclusionsAPS can significantly inhibit the migration and invasion ability of cervical carcinoma C-4I cells, which is related to the increase of E-cadherin expression and down-regulation of MMP-2 activity.