Effect Of Abnormal Expression Of MiR-363-3p On Biological Behaviors Of Human Hormone-independent Prostate Cancer Cells

Background:Prostate cancer has become one of the most serious threats to men’s health issues.With the aging of our population is increasing and improving of the treatment level, more patients with prostate cancer r can be earlie found.Moreover, gradually westernized lifestyle and diet also lead to the incidence of prostate cancer to increases substantially. Although castration as an important means of treatment for prostate cancer treatment can delay the progress of the disease, it can not avoid further deterioration of the castration-resistant prostate cancer and cancer metastasis to their organs.Therefore, exploring the pathogenesis of prostate cancer and finding out new treatments are more and more indispensable.More people focus on finding cancer-related genes in previous studies of prostate cancer and analysis the effects of mutations in prostate cancer cells’malignant phenotype and signaling pathways.Recently, researchers to explore the pathogenesis of cancer in depth from different angles, such as the promoter region of CpG methylation/ demethylation gene expression caused by silencing/activation; ubiquitination, phosphorylation and other protein post-translational chemical protein modification results in the activation/passivation; miRNA transcriptional regulation of target mRNA expression etc.Objective:To explore the expression of human prostate cancer cells and normal prostate epithelial cells and to investigate the impact of miR-363-3p prostate cancer cell phenotype.Methods:total RNA extracted from human prostate cancer cells Lncap,DU145 and PC3 and human normal prostate epithelial cells RWPE-1,SYBR Green real-time PCR(RT-PCR) was performed to detect the expression of miR-363-3p.Next, the miR-363-3p plasmid and miR-363-3p sponge plasmid was constructed to package recombinant lentivirus,while lentivirus pCDH-vector as a negative control,which was used to respectively infect DU145and PC3 cells, and RT-qPCR method was used to detect two cell lines in the expression of miR-363-3p.Then,After transfection,CCK 8 and Colony assay were employed to evaluate the effect of miR-363-3p on the cell proliferation;Transwell assay was used to detect changes of migration ability.The changes of tube formation ability were investigated by Microtubule assay in vitro.Results:the expression of miR-363-3p in the DU-145 and PC3 cells was significantly lower than that in the RWPE-1 cells (p<0.05).In vitro, compared with blank group, overexpression of miR-363-3p can significantly enhance the ability of proliferation,migration and invasion and microtubule formation human hormone-independent prostate cancer cells DU-145 and PC3(p<0.05),however,after downregulation of miR-363-3p corresponding biological phenotypes were significantly inhibited(P<0.05).Conclusions:miR-363-3p was upregulated in human prostate cancer cells. Over-expression of miR-363-3p can promote human prostate cancer cell ability of proliferation, invasion and migration and microtubule formation. In the process of prostate cancer, miR-363-3p may play a role of oncocancer gene, and is expected to become a new target for treatment of prostate cancer.