The Risk Factors Investigation And Clinical Significance Of Serum Hepcidin In The Diagnosis Of Infant Iron Deficiency Anemia

Objective In order to discuss the relationship between different risk factors and the severity of anemia, to strengthen the understanding of risk factors for infant iron deficiency anemia, 6~24 months iron deficiency anemia infants were analyzed. All of these mothers were Accepted questionnaire survey before treatment. Reasonable intervention measures starting from the mother during pregnancy will be formulated through these studies, the incidence of infant iron deficiency anemia in our province can also be reduce. Simultaneously, to detect the expression of serum hepcidin before and after treatment in infant iron deficiency anemia with or without vitamin D deficiency,and its relationship between serum ferritin, and explore its clinical diagnostic value and significance.Method A questionnaire including related influencing factors was investigated in 150 mothers, whose babies between 6~24 months were diagnosed for IDA from January to December in 2015. At the same time, 68 cases diagnosed during January to December were divided into two groups A and B. The group A consist of 20 IDA infants with vitamin D deficiency, group B consist of 48 IDA infants without vitamin D deficiency,the control group include 26 healthy infants. Blood counts included HB, MCV, MCH,MCHC, were assayed by hematology analyzer, the level of serum ferritin was assayed by chemiluminescence immunoassay, the levels of hepcidin and 25-(OH)D were assayed by ELISA.Results There were 115 cases between 6-12 months, accounting for 76.67%,significantly more than the age between 13-24 months. The univariate analysis showed that culture degree of mother, living area, production history, premature birth, low birth weight, breast feeding, timely introduction of solid foods, history of repeated infection or history of surgery and trauma were associated with the severity of anemia, a statistically significant difference(P<0.05). The multivariate logistic regression analysis showed that living area, whether pregnancy reaction, premature birth, breast feeding were associated with the severity of anemia. The levels of serum hepcidin in group A,group B and control group before treatments were(29.16±7.50)ng/ml,(27.11±7.10)ng/ml and(29.25±8.39)ng/ml, no significant difference(P>0.05). The levels of serum hepcidin in group A and group B after treatments were significant higher than control group [(36.21±5.68)ng/ml vs(29.25±8.39)ng/ml, P=0.000;(34.16±4.54)ng/ml vs(29.25±8.39)ng/ml, P=0.001]; but there are no significant different between group A and B(P=0.225). A positive correlation analysis between serum ferritin and hepcidin in group B both before and after treatments(r=0.352 and0.367, P=0.014,P=0.010).Conclusions IDA is still an important deficiency disease in infants of our country, the prevalence is associated with months of age, the smaller of age, the higher of IDA prevalence. Living in rural areas, low culture degree of mother, low family income are still high risk factors of infant IDA. Premature birth, low birth weight, breast feeding,timely introduction of solid foods, history of repeated infection or history of surgery and trauma are associated with the severity of anemia. The level of serum hepcidin was important in infant iron deficiency anemia both in diagnosis and treatment, but we should eliminate the interference effect of vitamin D deficiency.