Study On Antioxidative Activity Of The Fermented Codonopsis Lanceolata

ObjectiveIn recent years, with the rapid development of science and technology, people found that more than 90% of the disease closely related with active oxygen and free radical. Codonopsis lanceolata contained polyphenols, flavonoids, saponins, polysaccharides and many other functional components and mineral elements. It was high nutritional value and deeply loved by the people. In recent a few years, domestic and foreign scholars confirmed through a lot of research:Codonopsis lanceolata could protect liver, anti tumor, enhance immunity and had anti-oxidation effect. But there was no study on the antioxidant action of the fermented Codonopsis lanceolata components. Through the experiment in vivo and vitro, studied the fermented Codonopsis lanceolata of total saponins, total polysaccharides and not fermented Codonopsis lanceolata of total saponins, total polysaccharides antioxidation effect. We would selecte the functional components of Codonopsis lanceolata with strongest antioxidant effects to provide a theoretical basis for the development and utilization of Codonopsis lanceolata health care function.Methods①Take Codonopsis lanceolata cut,60℃ dried, crushed, and have 80 heads sieve. Weigh 1000g Codonopsis lanceolata and extract fermented (yeast) and non-fermented total saponins, total polysaccharides of Codonopsis lanceolata.②In vitro experiment, using DPPH radical scavenging capacity, oxygen radical absorbance capacity (ORAC), and cells antioxidant activity (CAA) experiment, selected functional components with optimal antioxidant capacity.③In vivo experiment,60 mice were randomly divided into blank control group, model group, positive control group, the fermented Codonopsis lanceolata of total saponins of low, medium and high dose groups. It was 10 mice in each group. Fermented Codonopsis lanceolata of total saponins of low, middle and high dose groups, were respectively given drugs 25,50,75 mg/kg·BW dose, once a day; black control group and model group were gavaged dH2O 0.1 mL/10mg·BW daily; positive control group were gavaged Vit C 10mg/kg·BW daily. It was gavaged for 30 days. After the last gavage, in addition to the black control group, the other 5 groups fasted for 16h (overnight), and then lavaged 1 time with 50%ethanol 12mL/kg·BW. The mice were recovered feed and water ad libitum. After 6h, take eye blood supernatant of the mice. Use enzyme-linked immunosorbent assay (ELISA) detection in serum of mice, glutathione (GSH) activity, protein carbonyl (PCO),8-hydrogen-oxygen ISO prostaglandin (8-iso-PGF2 a) contents and superoxide dismutase (SOD), heme oxygenase-1 (HO-1), NADPH quinone oxidoreductase (NQO-1) expression.Results①The DPPH radical scavenging activity results showed that:the fermented Codonopsis lanceolata total saponins group DPPH· scavenging activity was significantly higher than that in non-fermented Codonopsis lanceolata total saponins group, fermented and non-fermented Codonopsis lanceolata total polysaccharides group (P<0.05 or P<0.01). Comparison of fermented and non-fermented Codonopsis lanceolata total polysaccharides group DPPH· scavenging ability, were no significant difference (P>0.05). The fermented Codonopsis lanceolata total saponins group EC50 value was (109.52±0.05) μg/mL, non-fermented Codonopsis lanceolata total saponins group EC50 value was (123.81±0.04) μg/mL. Their EC50 values were greater than ascorbic acid group (40±0.01) μg/mL.②The oxygen radical absorbance capacity results showed that:the fermented Codonopsis lanceolata total saponins group oxygen free radical absorption capacity was significantly higher than that of non-fermented Codonopsis lanceolata total saponins group, fermented and non-fermented Codonopsis lanceolata total polysaccharides group (P<0.01). Fermented and non-fermented Codonopsis lanceolata polysaccharides group were compared, absorption capacity oxygen free radicals without significant difference CP>0.05).③ The intracellular antioxidant activity results showed that:the each dose group of fermented Codonopsis lanceolata total saponins group and non-fermented Codonopsis lanceolata total saponins group, cell fluorescence intensity compared with the black control group, the difference was statistically significant (P<0.05 or P<0.01); Fermented and non-fermented Codonopsis lanceolata total polysaccharides group of concentration 150,200,250 μg/mL dose group compared with the black control group, there were significant differences (P<0.05). Fermented Codonopsis lanceolata total saponins group inhibition of ROS oxidation of DFCH (P<0.05) had the strongest ability.④The fermented Codonopsis lanceolata total saponins of low, middle and high dose group, the activity of GSH were significantly higher than that in model group (P<0.05 or P<0.01).⑤The fermented of Codonopsis lanceolata total saponins of low, middle and high dose group, PCO and 8-iso-PGF2a contents were significantly lower than that in model group (P<0.05 or P<0.01).⑥The fermented Codonopsis lanceolata total saponins of low, middle and high dose groups, SOD, HO-1 and NQO-1 expression were significantly higher than that in model group (P<0.05 or P<0.01).⑦The fermented Codonopsis lanceolata total saponins high dose group and positive control group, comparison of GSH activity, PCO、8-iso-PGF2a contents and the expression of SOD、HO-1、NQO-1, the difference was not statistically significant (P>0.05).Conclusions①Fermented, non-fermented Codonopsis lanceolata total saponins and fermented, non-fermented Codonopsis lanceolata totale polysaccharides have antioxidant activity. Fermented of Codonopsis lanceolata total saponins antioxidant activity were better than that of the non-fermented total saponins and fermented, non-fermented Codonopsis lanceolata total polysaccharides.②Fermented Codonopsis lanceolata total saponins can enhance the activity of antioxidant enzymes, reduce the protein oxidative damage and lipid peroxidation damage in vivo, and promote antioxidant protein expression.