Studies On Quality Evalution Of Uygur Medicine Galls Of Quercus Infectoria Oliv.(Fagaceae) And Its Inhibitory Effect On Streptococcus Mutans

Objective: Through the research of Uygur medicine galls of Q. infectoria Oliv. evaluation of quality standards, three kinds of chemical components of qualitative and quantitative analysis, the content of trace elements determination and its effect on Streptococcus mutans reactive and reliable scientific basis and research methods are provided for the control of the quality of Uygur medicine galls of Q. infectoria, extensive development and utilization of the Uygur medicine galls of Q. infectoria resources. Methods: According to the Pharmacopoeia of the people’s Republic of China and literature character identification of galls of Q. infectoria; It’s powder for microscopic identification, it’s water extract, ethanol extract, and petroleum ether extract of the systematic pre experiment. The 10 batches of galls of Q. infectoria content of moisture, ash, water soluble extract and ethanol soluble extract were determined. Determine the content of total polyphenol in galls of Q. infectoria by vis-spectrophotometry. Using thin layer chromatographic(TLC) to qualitative analysis gallic acid, methyl gallate,and ellagic acid and simultaneous using high-performance thin-layer chromatographic(HPTLC) quantitative analysis for allic acid, methyl gallate,and ellagic acid in galls of Q. Infectoria. Using Thin-Layer Chromatography-2,2-Diphenyl-1-Picrylhydrazyl to screen antioxidant component in 10 batches of galls of Q. infectoria and comparison of different batches of galls of Q. infectoria antioxidant capacity. The use of high performance liquid chromatography(HPLC) techniques to analyze three chemical constituents in galls of Q. Infectoria. The content of trace elements(As, Hg) of 10 batches of galls of Q. Infectoria were determined by atomic fluorescence spectrometry. The content of trace element(Cu) of batches of galls of Q. Infectoria were determined by iductively cupled pasma-aomic eission sectrometry. The content of trace elements(Pb, Cd) of 10 batches of galls of Q. Infectoria were determined by graphite furnace atomic absorption spectroscopy. The 96 pore plate method of 10 batches of galls of Q. Infectoria inhibitory effects on the activity of Streptococcus mutans. Results: The result of systematic preliminary test showed that the galls of Q. Infectoria may contained polypeptide, protein, sugar, polysaccharides, glycosides, organic acids, phenols, tannin and other ingredients; The moisture, total ash and acid insoluble ash, water solube extract and alcohol soluble extract of 10 batches galls of Q. Infectoria were 9.18%、1.62%、0.12%、71.22% and 76.24%, respectively. Total polyphenols of 10 batches of galls of Q. Infectoria were ranged from 513.7549–724.5982 mg/g. The optimized TLC condition is perform on silica gel GF254 TLC plates with toluene-ethyl acetate-formic acid(6:4.5:2, volume ratio) as mobile phase, With this method, the galls of Q. Infectoria from 10 batches were analysed and found most compounds were the same, but different from the contents. The gallic acid,methyl gallate, and ellagic acid of 10 batches of galls of Q. Infectoria were 2.8397 、 3.6146 and 3.1664 mg/g, respectively. Using Thin-Layer Chromatography-2,2-Diphenyl-1-Picrylhydrazyl to screen antioxidant component in 10 batches of galls of Q. infectoria that contain at least four antioxidant components, including ellagic acid, gallic acid, and methyl gallate etc, but different from the antioxidant capacity. The use of HPLC techniques to analyze three chemical constituents in galls of Q. Infectoria, and the content of gallic acid, methyl gallate, and gallic acid were 50.0086、119.8675 and 14.3981 mg/g, respectively. The content of trace elements(As、Hg、Cu、Pb、Cd) of 10 batches of galls of Q. Infectoria were ranged from 0.0707-0.1959、0.1012-0.1652、3.7467-12.4358、0.0098-0.0855 and 0.0048-0.0176 μg/g, respectively. The 10 batches of galls of Q. Infectoria on Streptococcus mutans growth and biofilm formation of Streptococcus mutans had certain inhibition, but different from the inhibition rate. Conclusion: This study explored the character identification, microscopic identification, and the systematic pre experiment. The content of moisture, total ash and acid insoluble ash, water solube extract and alcohol soluble extract of 10 batches galls of Q. Infectoria were checked. The content of total polyphenols of 10 batches of galls of Q. Infectoria were determined. Using HPTLC quantitative analysis for allic acid, methyl gallate,and ellagic acid in galls of Q. Infectoria and simultaneous using TLC-DPPH to screen antioxidant component, the method combined the separation and evaluation of active chemical composition, and combined the changes of chemical compositions of fingerprint of herbs and their biological activities. Thus it established the spectrum effect relationship of the fingerprint of herbs in the real way, providing a scientific basis for the quality control and evaluation of the effects. Using of HPLC techniques to analyze three chemical constituents in galls of Q. Infectoria. Trace elements of 10 batches galls of Q. Infectoria were determined. To provide scientific basis for the establishment of a scientific quality standard, the galls of Q. Infectoria resources get widely developed and utilized. At the same time, preliminary study of galls of Q. Infectoria inhibiting activity of Streptococcus mutans by 96 well plate method, for deeper step research laid the foundation.