| Fluorescent nanomaterials with unique advantages show a great application prospect in early detection and screening of tumor cells. Hyaluronic acid(HA), a hyaluronan composed by disaccharide units containing N-acetyl-D-glucosamide and glucuronic acid, exists widely in the fungi, plants, insects, humans and vertebrates. The molar mass of HA ranges between 105 and 108 Da. It plays important physiological roles of HA in living organisms including maintenance of viscoelasticity of liquid connective tissues(i.e. the synovial fluid in the joints or in the vitreous humor), the control of the tissue hydration, water transport, tissue repair, and various receptor-mediated functions in cell detachment, tumor development and inflammation.When the tumor is treated by antineoplastic drugs, it will produce certain side effect to human body, such as cardiac toxicity, liver toxicity, marrow toxicity.Daunorubicin hydrochloride(DNR) belongs to erythromycin anthraquinones and is one of nonspecific antitumor drugs. It can embed DNA strand to block the synthesis of DNA and RNA. Daunorubicin hydrochloride is used in the treatment of leukemia, malignant lymphoma, etc. But the heart will suffer from a dose dependent irreversible toxicity, marrow toxicity. Rutin, known as vitamin P, as the natural flavonoid derivatives, shows many biological activities, such as antioxidant, hemostatic, antitumor and antibacterial agent, and are widely used in clinical medicine. Such flavonoids compounds also can be used as highly effective inhibitors by adjusting the concentration and source of free radicals. If we can effectively monitor the drug concentrationin the process of tumor treatment, we would maximum the antitumor drug efficacy and reduce their side effects.In this paper, we developed chemical biosensor based on core-shell nanomaterialsand mesoporous materialsand gold nanoparticles with the excellent physical and chemical properties. The detailed materials including three major components are summarized as follows:1. Early detection of cervical cancer cells based on hyaluronic acid modified fluorescent nanoparticlesThe fluorescent(FITC) nanoparticles were synthesized using a water-in-oil(W/O) micromulsion technique and characterized by TEM, Fourier infrared, and Fluorescence spectrophotometer. The fluorescent silica core-shell nanoparticles were modified with hyaluronic acid targeting at he overexpressed protein receptors CD44 in the cell surface of the cervical cancer Hela cells. The specific binding of hyaluronic acid-nanoparticles and CD44 cells was characterized by laser scanning microscopy(LSM). The results showed that the method offered potential advantages of sensitivity and simplicity to cervical cancer cells comparedwith the traditional method of fluorescent molecular markers, and it is expected to provided an alternative method for the detection of cervical cancer cells.2. Daunorubicin hydrochloride electrochemical sensor Based on Glassy Carbon Electrode Modified with MPs(FDU-15), Au Nanoparticles and HS-β-CDUsing three block copolymer F-127 as the template agent, an orderly mesoporous phenolic resin polymer MPs(FDU-15) was prepared by the method of Evaporation-Induced Self Assembly(EISA), and the resulting product was characterized through XRD, TEM. A Daunorubicin hydrochloride electrochemical sensor was fabricated by immobilizing MPs(FDU-15)/Au nanoparticles composite and HS-β-cyclodextrin on the surface of glassy carbon electrode. The electrochemicalimpedance spectroscopy(EIS) and cyclic voltammetry(CV) were used to study the electrochemical behavior of modified electrodes. Based on beta cyclodextrin and the embedded role of anthraquinone in package, the sensor showed good selectivity and current response towards daunorubicin hydrochloride due to the unique conductivity of nanometer gold. Under the optimal experimental conditions, the current is linear with the concentration of daunorubicin hydrochloride in the range from 9.5 × 10-7- 2.6 × 10-6 mol L-1(r = 0.9968), 2.6 × 10-6- 4.0 × 10-5 mol L-1(r = 0.9912), and the detection limit was 1.1 × 10-8 mol L-1(S/N= 3).3. Rutin Biosensor Based on Glassy Carbon Electrode Modified with Au Nanoparticles, L-Tyrosine, HOOC-SBA-15, and polyphenol oxidaseThe carboxyl functional mesoporous silica materials SBA–15 was characterized by XRD, TEM, Fourier transform infrared. And the polyphenol oxidase was fixed to the electrode surface under the action of EDC and NHS to prepare the rutin biosensor. The electrochemical behavior of modified electrodes was studied by EIS and CV. The modification of carboxyl mesoporous silica and gold nanoparticles can increase the effective surface area of the chemical sensor. Results showed that the modified electrodes exhibited a good current response to rutin and good anti-interference ability. Under the optimal experimental conditions, the current was linear with the concentration of Rutin in the range from 2.5 × 10-6- 7.2 × 10-5 mol L-1, with a detection limit of 3.1 × 10-7 mol L-1(S/N = 3) and a correlation coefficient of 0.9947. |
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