Endometrial HOXA10 Expression In Mice Undergoing Asynchronous Embryo Transfer

Background Rapid development of assisted reproductive technology has overcome too many infertility factors, but implantation failure results in still low pregnancy success of in vitro fertilization- embryo transfer (IVF-ET). Now about two-thirds of the pregnancy failure is considered related to asynchronous transfer. Although there is not yet conclusive evidence that asynchronous transfer during human IVF-ET will increase the transplant offspring malformation rate, but animal studies indicate that adverse effects will result in asynchronous transfer including implantation failure, miscarriage, malformation or abnormal embryonic development process. Therefore study on the mechanism of the impact of asynchronous transfer on pregnancy related is of great significance for improving outcome of IVF-ET. Some marks are needed to measure the receptivity of endometrium in order to study the change. Homeobox gene A10 (HOXA10) is one marker of endometrial receptivity. Integrin 03 (ITGβ3) is a downstream target gene of HOXA10, and insulin-like growth factor binding protein-1 (IGFBP-1) is the marker molecule of decidualization. Therefore, we detect the expression level of these three factors to investigate the mechanism of asynchronous transfer changing endometrial receptivity.Objective To investigate whether asynchronous transfer by changing the expression of HOXA10 and other endometrial receptivity associated markers and changes the endometrial receptivity. Verifying planting window adapting induced by asynchronous transfer is pathological abnormalities.Methods Select 6-8 weeks health ICR mice, collecting ovarian stimulation pregnant mice D2 embryos oviduct located within the 2-cell embryos were randomly divided into two parts, one to be kept frozen; Another part continue to culture until D3 embryos then frozen. Ligation mice mated female mice, select false pregnancy D3 mice, recovering D2, D3 mouse embryos, the embryos recovered at different times, are transfered respectively to the left and right oviduct of the pseudopregnant mice, formatting synchronous and asynchronous transfer self-control. Excise the mouse uteri tissue on D5, Using Chicago blue staining to determine the number of implantation site, HE staining to observe endometrial pathology, RT-PCR to detect HOXA10, ITGβ3, IGFBP-1 mRNA expression and Western blot to detect the expression of related proteins and.Results Asynchronous transfer significantly reduces embryonic implantation rate (P=0.002), Comparing to synchronous transfer group, endometrial secretory in asynchronous transfer group response poorer and develop slower. Endometrial receptivity related gene HOXA10, TTGβ3, IGFBP-1 mRNA expression, and the corresponding protein expression are more decreased in asynchronous transfer group comparing to synchronous transfer group.Conclusions Asynchronous transfer reduces endometrial receptivity and declines the embryo implantation rate through reducing the expression of HOXA10, ITGβ3, IGFBP-1.