Marine Mussel Inspired Chemistry For Self-polymerized Coatings And Study On Biocompatibility

With the development of modern medicine and biotechnology, a growing number of materials and devices have been used for research interventional device; controlling the interfacial chemical and physical properties, modulating the behaviours of cells and biomolecules on materials surfaces, improving the biocompatibility become the most foundation problem for development of high-performance biomaterials and devices.Inspired by the adhesion strategy of marine mussels:dopamine, we constructed various typical catecholamine films, namely poly(dopamine) (pDA), poly(L-DOPA) (pLD), poly(norepinephrine) (pNE) under alkaline aerobic condition via dopamine, L-dopa, and norepinephrine as monomers. Through modulating the reaction conditions to optimize the deposition parameter, research the mechanism, hemocompatibility and biocompatibility of these films. (1) Through Ellipsometer, Atomic Force Microscope (AFM) and Water contact angle (WCA) to test the physical properties of films, the results confirmed the successful deposition of these catecholamine films onto 316L stainless steel (SS), compared with 316L SS, the physical properties have a great change, and these films were also different with each other, they all have a better hydrophilic and roughness, pNE film was suitable as a coating of bio-materials with good deposition rate, roughness and hydrophilic. (2) Utilize AO II, micro-BCA, UV-vis spectra, Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS) and Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) to analysis the chemical properties. AO II and micro-BCA illustrated that the three films have different-OH and-NH2 grou; UV-vis, FTIR and XPS indicated that the films all detected the characteristic peak, C, N, O high resolution spectrum of XPS indicated different C, N, O combination forms and element ratio, and further reflected Tris was involved in the reaction; MALDI-MS helped to give a further structural investigation and allowed us to propose the structure model for the monomer building blocks. (3) Testing the hemocompatibility of the films, pDA film had a bad blood compatibility because of more -NH2, pLD and pNE films inhibited platelet adhesion in a certain extent because of carboxyl, hydroxyl and quinone. (4) ECs proliferation and migration culture, MSC, OB proliferation test presented the desired cellular responses, provided a good growth environment for cells, in the three kinds of thin film, pNE is the best; the macrophages experiment result manifested that compared with 316L SS and pDA film, pLD and pNE films didn’t show the ability to resist inflammation.All the researches show that pDA, pLD, and pNE have a good performance in blood compatibility and hemocompatibility. This one-step formation of catecholamine films will make a significant contribution in the area of surface engineering and implantable materials, and make a foundations for the modification of biomolecules subsequent.All the researches show that pDA, pLD, and pNE has a good performance in blood compatibility and hemocompatibility. This one-step formation of catecholamine films will make a significant contribution in the area of surface engineering and implantable materials, and make a foundations for the modification of biomolecules subsequent.