The Preparation Of Fe₃O₄ Fluorescent Magnetic Composite Functional Materials

Photodynamic Therapy(PDT) has attracted extensive research interest in field of cancer.treatment. Compared with the conventional cures for cancer, photodynamic therapy has the characteristics of small trauma, wide therapeutic scope and good applicability, and it can be combined with surgery, radiation therapy, chemical treatment for cancer treatment. PDT has three essential elements: photosensitizer, light source and reactive oxygen species. Photosensitizer is the most important factor in photodynamic therapy. Generally, the optical properties of the photosensitizer greatly influences the light irradiation wavelength, and the photochemical properties of photosensitizer affect the active oxygen quantum yield. To develop new photosensitizer for PDT, we designed and synthesized a novel pyropheophorbide-a(PPa) directly modified magnetofluorescent nanoparticle(Fe3O4@SiO2@APTES@PPa, MFNP) for photodynamic therapeutics based on Fe3O4 and PPa core-shell structure, aiming for magnetic targeting.This paper carries on the research in two parts. In first part, the preparation of Fe3O4@SiO2@APTES@PPa was displayed. The core-shell nanoparticle(NP), Fe3O4@SiO2@APTES was firstly prepared, then Chlorophyll is coated on the surface of NP. The morphology and physical properties are characterized and analyzed by XRD, TES, IR, UV, TGA, VSM and so on. MFNP has good water solubility and fat solubility, strong absorption in 670 nm. After it was excitated by the light of 422 nm, a strong red fluorescence emission at 700 nm was observed. Fe3O4@SiO2@APTES@PPa is expected to be used in Photodynamic therapy, magnetic resonance imaging, and biological probe. In second part, nine new chlorin derivatives were designed and synthesized by modifying the structure of pyropheophorbide-a. Their chemical structures were characterized by elemental analysis, UV, IR, 13 C NMR and 1H NMR spectra.