| Objective:To observe effects on rat oxygen consumption, membrane potential, adenylate content,uncoupling protein 2(UCP2) by different doses of aconite and to study mechanism to promote energy metabolism of aconite preliminary.Methods:The 50 SPF level SD rats were divided randomly into five groups—the control group, the positive control group(group of thyroid hormone), the high doses of aconite group, the middle doses of aconite group, and the low doses of aconite group.Every group has 10 rats. Saline 1ml/100 g was lavaged with the blank group,levothyroxine sodium solution 100μg/100 g was lavaged with the thyroid hormone group, aconite decoction 1.05g(crude drug)/100 g was lavaged with the high doses of aconite group, the aconite decoction 0.525g(crude drug)/100 g was lavaged with the middle dose of aconite group, and the aconite decoction 0.263g(crude drug)/100 g was lavaged with the low doses of aconite group. All the rats was lavaged once a day for 30 days.Then took off their necks to death, extracted a portion of liver tissue and mitochondrial to determined mitochondrial oxygen consumption, membrane potential,the adenylate contents and UCP2 expression of each group.1. Mitochondrial oxygen consumption determination experiment: compared the blank group with the thyroid hormone group, the mitochondria protein content was significantly decreased. Mitochondrial state 3 respiration(ST3), mitochondrial state 4respiration(ST4), and oxidative phosphorylation rate(OPR) were significantly increased, respiratory control rate(RCR), and phosphorus oxygen ratio(P / O) were significantly decreased; the high, the middle and the low aconite dose groups of mitochondrial protein contents were significantly increased; ST3 of the high and the middle dose aconiti lateralis preparata groups decreased significantly, the low aconite dose group had no significant difference; ST4 in the high dose group decreased significantly, the low dose group had no significant difference; RCR,P/O and OPR of ther high and the middle dose groups were significantly lower, the low dose group had no significant difference. Compared with thyroid hormone group, mitochondrial protein content of the low dose group increased significantly, ST3 and ST4 were significantly decreased; RCR of the low dose group increased significantly, the aconite in the high and the middle dose groups had no significant difference; P/O and OPR of the high, the middle and the low dose groups significantly reduced.Compared with the low dose of aconite group, aconite high, middle dose group of mitochondrial protein contents were significantly increased in the, ST3 were significantly lower,ST4 aconite high dose group decreased significantly, aconite dose group no significant difference; aconite is high, middle dose group of RCR, p/o, the OPR significantly reduced.2. Determination of mitochondrial membrane potential: compared with the blank group, membrane potential of the thyroid hormone group decreased significantly,membrane potential of the high and the low dose groups increased significantly, the low dose group had no significant difference; compared with the thyroid hormone group, membrane potential of the high and the low dose groups were significantly increased.Compared with Aconite aconite high low dose group, middle dose group MMP increased significantly.3.The content of mitochondrial adenine nucleotides: compared with the blank group, there was no significant difference in the content of ATP and AMP in the thyroid hormone group, the content of ADP was significantly increased, and the totaladenine nucleotides was not significantly different. ATP content of the high and the medium dose groups increased significantly, there was no significant difference in the low dose group; ADP of the high, the middleand and the low dose of groups were significantly increased, there were no significant differences in the content of AMP;Aconite total adenylate content of the high and the medium dose groups increased significantly, there was no significant difference in the low dose group. Compared with the thyroid hormone group and the high dose group, ATP content increased significantly, and there was no significant difference in the low dose group; ADP content of the low dose group decreased significantly, there was no significant difference in the high dose group; AMP content of the high,the middle and the low does groups had no significant difference; Aconite total adenylate contentin the high and the medium dose groups increased significantly, and there was no significant difference in the low dose group.Compared with the low dose of aconite group,aconite, ATP content in high and middle dose group increased significantly; aconite high dose group of ADP content increased significantly, aconite dosage group no significant difference; aconite, AMP content in high and middle dose group had no significant difference; aconite is high, middle dose group, the total adenylate content were significantly increased.4.Mitochondrial UCP2 expression measurement experiment: compared with the blank group, UCP2 expression of the thyroid hormone group increased significantly,UCP2 expression of the high, the middle and low dose groups of were enhanced, but no significant difference. Compared with the thyroid hormone group, UCP2 expression of the high, the middle and the low dose groups were significantly decreased.Compared with Aconite aconite high low dose group, there were no significant differences in the dose group.Conclusions:1.The aconite can increase the ST3 and ST4 mitochondrial oxygen consumption to enhance energy metabolism;2.The aconite can increase mitochondrial membrane potential to prepare full freedom for ATP synthesis.3.The aconite can increase mitochondrial ATP content significantly and prepare a large amount of energy for organism life activity;4.Although aconite doesn’t increase thermogenesis directly by increasing UCP2 expression, but it can make more H+ through UCP2 returning to increase the body thermogenesis;5.The dose of the aconite and its promotion of energy metabolism showed a certain correlation. The high and the middle dose groups can promote energy metabolism in rat liver mitochondria. |
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