Influence Of Endometrial Receptivity On By Integrin αvβ3 And Osteopontin(OPN) Expression In Women With Elevated Serum Progesterone (P)and/or Oestradiol (E2) Who Are Undergoing In Vitro Fertilisation (IVF)

Assisted reproductive technology (ART) is currently the most effective method for the treatment of infertility. It is a new area of multidisciplinary, involving multiple disciplines of obstetrics, gynecology, andrology, genetics, embryology and molecular biology, explored the mysteries of reproduction, achieved human self-regulation of human reproduction. In vitro fertilization-embryo transfer (IVF-ET) as the most representative of the ART technique was published in the 1980s. Controlled ovarian hyperstimulation is the precondition of IVF, embryo quality and endometrial receptivity is the key to successful implantation. However, during IVF cycle, endometrium and embryo are exposed to supra-physiological concentrations of oestradiol and progesterone under ovarian hyperstimulation, which could affect endometrial receptivity, so that the pregnancy rate may decline. Endometrial receptivity simply refers to the endometrium for embryo receptivity. A large number of architectural, cellular, biochemical and molecular events occurred in the endometrium within the implantation window (i.e.6-8 days after ovulation). Integrins superfamily is a group of molecules that have expressed characteristicly. Integrin is a surface transmembrane receptor composed of alpha and beta subunits. It links cells,the extracellular matrix and cell adhesion. Sametimes,it mediates and integrates information transfer of internal and external. Each subtype of the integrin family exhibiting a certain rule of time and the distribution of specific space when expression. Subtype of integrin avβ3 is the most widely extracellular matrix receptors. Integrin avβ3 and its extracellular matrix ligand, osteopontin (OPN), are two of the best characterised endometrial receptivity biomarkers. We use these two markers of the endometrial receptivity that expressed in the patients with high estrogen and progesterone within IVF as the experimental evidence to determine endometrial receptivity.Objective:To explore whether endometrial receptivity is determined by osteopontin(OPN) and integrin avβ3 expression in women with elevated serum progesterone (P)and/or oestradiol (E2) who are undergoing in vitro fertilisation (IVF). Methods:According to serum hormone levels on the day of HCG administration,33 Infertilewomen were divided into 3 groups:the high E2, high P, and high E2 and P groups.The control group included 11 fertile, healthy women. Endometrial biopsy was performed on ovulation day +7 to +8 for all study participants, and the mRNA and protein expression levels of OPN and integrin αvβ3 were analyzed. Result:No statistically significant differences regarding OPN and integrin αvβ3 expression were found between infertile patients in the high P, high E2, high E2 and P and control groups. There was no significant correlation between OPN and integrin αvβ3 staining intensity during the implantation window biopsy in any of the groups studied. Conclusion:Endometrial OPN and integrant αvβ3 expression/co-expression is not impaired during the window of implantation in patients with high P, high E2, or high E2 and P levels. The clinical value of assessing endometrial receptivity with OPN and integrin αvβ3 seems to be uncertain.