The Animal Study On Effect Of Growth Hormone On Endometrial Receptivity And Immunoregulation

Backgroud:In recent decades, human assisted reproductive technology has made great strides, although the clinical pregnancy rate is still unsatisfactory,[1] due to the uncertainties associated with embryo implantation. Endometrial tolerance is critical to successful implantation. Enhancing the endometrial receptivity has therefore emerged as a hot research topic in the field of reproductive medicine. Growth hormone (GH) is an important peptide hormone secreted by the pituitary gland. It modulates reproductive function by directly binding with growth hormone receptor in the target gland or indirectly inducing the formation of IGF- I. [2]. GH modulates the ovarian function, and can improve ovarian response in the presence of GnRHa for controlled ovarian stimulation for quality egg production, higher fertilization rate, viable embryos and higher pregnancy rate[3,4] by improving oocyte quality and endometrial receptivity , but few researches revealed its real mechanism.Besides,embryo implanting in endometrium is a complicated process involved the regulation of immune tolerance,and Th1/Th2 type celluar factors play an important role in the formation of immune tolerance[5,6].In the field of surgery,researchers have found that GH can regulate the immune function[7,8].So explore whether GH can regulate immune tolerance and improve endometrial receptivity in"implantation window"or not is helpful for providing a theoretical basis for choosing medicine to cure some tough diseases,such as unexplained recurrent implantation failure(RIF).( - ) Explore the effect of growth hormone on endometrial receptivity in SD MouseObjective: To study the effect of growth hormone (GH)on expressions of integrinαvβ3 (ITGαvβ3), osteopontin (OPN) in Sprague–Dawley (SD) mouse and the strategies to improve the endometrial receptivity.Methods:Fifty mice were divided into two groups randomly. The control group was subcutaneously injected with NS in its diestrum stage while the experimental group was administered GH.The expression of ITGαvβ3 and OPN in the two groups was detected by immunohistochemistry techniques and real time PCR techniques in mouse endometrium on 4th day after mating.Results.:1,The expression of ITGαvβ3 and OPN in endometrium was showed in cytoplasm and cell membrane in both groups.2,A stronger expression of ITGαvβ3 and OPN was found in the GH group compared with the controls,and the difference was statistically significant(P<0.05). 3,A stronger expression of mRNA(ITGαvβ3) and mRNA ( OPN ) was found in the GH group compared with the controls,and the difference was statistically significant (P<0.01).Conclusion:GH can enhance the expressions of ITGαvβ3 and OPN in implanta- tion endometrium of mouse. Use of GH can improve the endometrial receptivity.(二)Explore the effect of growth hormone on immunoregulation in SD MouseObjective:To study the effect of growth hormone on immunoregulation in"implanatati- on window". Methods:Fifty mice in experiment(-) were taken blood in caudal vein respectively in diestrum stage before nothing injected and on 4th day after mating. The expression of IL-2 and IL-4 in each blood was detected by ELISA techniques.Results:1,There was no statistical significant of the expression of IL-2 and IL-4 before/after mating between two groups .2,In each group, the expression of IL-2 after mating was weaker than that before mating while the expression of IL-4 was stronger, and the difference was statistically significant(P<0.01).Conclusion:The balance of Th1/Th2 type celluar factors converted to the inclination of Th2 type celluar factors was related to the formation of immune tolerance in implantation window,and growth hormone can not regulate it in healthy SD mouse.