The Effect Of Rivaroxaban On Coagulation Function In Rabbits And The Study Of Its Mechanism

Objective: To observe changes of APTT,PT,TT,anti-Xa activity and the blood coagulation factors(XII,XI,IX, VIII,VII,V,X,and II)in the different doses of rivaroxaban to facilitate understand the effect of rivaroxaban on coagulation system and its mechanism preliminarily, also hope to have a certain reference value for clinical medication.Materials and methods: select 30 size, body weight without significant difference and was not used for any other experimental rabbits and to oral rivaroxaban doses were randomly divided into 0.3mg/kg, 1.0mg/kg,3.0mg/kg, 10.0mg/kg and normal controls, 6 rabbits in each group.Blood was taken from the exposed femoral artery.Observed the change of PT, APTT, TT anti-Xa and the blood coagulation factors(include XII,XI,IX, VIII,VII,V,X,and II)in 2 hours of oral rivaroxaban.Using the paired t test, SNK-q variance analysis, Spearman rank correlation analysis and other statistical methods to analyze the experimental data, to understand the impact of rivaroxaban on the monitoring indicators, and preliminary study of its impact mechanism.Results: 1, The PT, APTT and TT level before taking medicine the variance analysis showed no significant difference between the groups(P > 0.05); 2, After taking the drug control group, 0.3mg/kg group and 1.0mg/kg group between PT showed no significant difference(P=0.322), and 3.0mg/kg and 10.0mg/kg group respectively with the other 4 groups. The difference was significant(P < 0.01); APTT is only in the 10.0mg/kg group and the other 4 groups have significant difference(P < 0.01); the TT index showed no significant difference(P=0.864); 3, Each PT in the 3mg /kg dose group medication time began to extend the statistically significant(P<0.05) begin to extend the time has significant differences in the dose(P<0.05); while TT has had no significant effect; anti Xa activity in the 0.3mg/kg dose group had significantly increased(P<0.01). 4, PT, anti Xa activity and rivaroxaban dose showed a linear correlation(PT:r=0.74, P<0.05 vs Anti-Xa: r = 0.97, P <0.01); APTT and rivaroxaban dose correlation is not obvious(r=0.201, P= 0.098); 5, 0.03mg/kg dose group of PT after medication and controls according to compare changes had no significant difference(9.65±2.73vs9.71±2.44,P=0.34); and anti-Xa is obviously have statistical significance(4.83±2.12 vs 12.88±2.48,P<0.01); 6, Compared with that before treatment, in all the experimental groups of coagulation factor activity decreased. In the 1mg/kg dose group began to reduce the beginning of FXa with statistical significance(P<0.05), and the rest of the 3mg/kg dose group changes in the beginning with a statistically significant difference(P < 0.05).Conclusions: 1, After taking the rivaroxaban, the PT, APTT and anti-Xa were prolonged; 2, There was a linear correlation between the PT and the anti-Xa and the dose of the rivaroxaban, and the correlation that of the anti-Xa was better than PT; 3, the anti-Xa may be more suitable for clinical monitoring of the effects of anticoagulant of rivaroxaban; 4, After taking rivaroxaban the most of coagulation factors activity are decreased...