| Objective:Profiling the expression pattern of LncRNAs using a microarray method in a contusion spinal cord injury(SCI) mouse model.Method: 1. ICR mice(SPF, 20-25g), After the mice were deeply anesthetized with intra-peritoneal pentobarbital sodium(50 mg/kg body weight), the spinal cord was contused with a Multicenter Animal Spinal Cord Injury Study(MASCIS) Impactor weight-drop device, which uses a 10-g weight impact rod dropped from a height of 25 mm to produce a reliable contused SCI model2. The spinal cord located 5 mm proximal and distal to the injury epicenter was removed for RNA extraction and Profiling the expression pattern of LncRNAs using a microarray method in a contusion spinal cord injury(SCI) mouse model.3. Analysis the expression pattern ofLncRNAs with the bioinformatics method.Results: 1. thecontusion spinal cord injury(SCI) mouse mode were reliable by the MASCISImpactor weight-drop device. RNA concentrations were determined based on the absorbance at 260 nm, and quality control standards at A260/A280 = 1.8–2.1 using a SMA3000(Mertion, Beijing).2. Compared with a spinal cord without injury, few changes in LncRNA expression levels were noted 1 day post-injury. The differential changes in LncRNA expression peaked 1 week after SCI and subsequently declined until 3 weeks post-injury.3. Gene ontology(GO) analysis indicated that differentially expressed mRNAs were involved in transport, cell adhesion, ion transport, and metabolic processes, among others. Kyoto Encyclopedia of Genes and Genomes(KEGG) enrichment analysis showed that the neuroactive ligand-receptor interaction, the PI3K-Akt signaling pathway, and focal adhesions were potentially implicated in SCI pathology. We constructed a dynamic LncRNA-mRNA network containing 264 LncRNAs and 949 mRNAs to elucidate the interactions between the LncRNAs and mRNAs.Conclusion: Overall, the results from this study indicate for the first time that LncRNAs are differentially expressed in a contusion SCImouse model. |
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