| Objective: To study the effects of Liensinine on Poliferation and cell cycle of human bladder cancer 5637 cells. Preliminary discuss the mechanism of the impact of liensinine on 5637 for cell cycle Methods: Treat bladder cancer cell line 5637 with Liensinine solution, the concentration were(1.562, 3.125, 6.250, 12.500, 25.000 μg/mL) respectively. CCK-8 kit and the colony formation test was done to detect cell viabilities after treatments and then inhibition rates were calculated. Flow cytometry was used to detect the effects of Liensinine on cell cycle of 5637 cells after the treatments. Western blot was used to detect CDK2 expression. Results: CCK-8 assay indicated that with the increasing concentration of the drug and prolonged incubation time, Liensinine inhibited the 5637 cell proliferation significantly. After treatment of 48 h, the proliferation inhibition rates were(7.18±0.05)%,(12.15±0.04)%,(19.21±0.01)%,(23.96±0.05)%,(30.45±0.03)%. The difference was statistically significant(p<0.05). Colony formation test also showed that Liensinine could inhibit the proliferation of 5637 cells. The proliferation inhibition rates were(40.07±0.02)%,(55.66±0.05)%,(72.00±0.01)%,(85.59±0.01)%,(93.1±0.01)%. Flow cytometry indicated that the inhibitory effect reached its maximum at 48 h post-treatment, G1-phase cells of bladder cancer 5637 had a significant increase and share of possession were(1.562 μg/m L, 55.03±0.81)%,(3.250 μg/m L, 56.97±0.69)%,(3.250 μg/m L, 57.87±0.11)%,(12.500 μg/m L, 58.00±0.54)%. Interestingly, we also found that Liensinine(25.000 μg/m L) could arrested 5637 cells at S-phase.The S-phase cells of bladder cancer 5637 had increased and share of possession was(41.51 ± 0.62)%. Western blot showed that the expression of CDK2 in dosing groups had down-regulated significantly. Conclusions: Liensinine could inhibit the proliferation of 5637 cells significantly, arrests cells at G1 phase and S phase, and the mechanism may have a close relationship with the down-regulation of CDK2. |
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