Effect And Mechanism Of Vitexin In Inhibition Of Proliferation Of SMMC-7721 Cancer Cell

OBJECTIVE: To investigate the anti-proliferative effects of Vi texin on the growth of human hepatocelluar carcinoma cell line SM MC-7721 in vitro and its molecular mechanism.METHODS: SMMC-7721 cells were cultured in vitro by diffe rent concentrations of Vitexin, the cytotoxic effect of drugs was me asured by MTT assay at the time of 48, 72, 96 h. SMMC-7721 cell s were cultured by different concentrations of Vitexin(0, 25, 50, 100μmol·L-1) after 72 h, the morphological changes of apoptosis of cell were determined by Hoechst33258. Cell apoptosis and mitochondri al membrane potentia(ΔΨm) were identified by flow cytometry(F CM). The expression level of P53、Bcl-2、Bax and other apoptosisrelated genes were analyzed by q RT-PCR. The expression level of p53、Bcl-2、Bax and other apoptosis-related proteins were analyzed by Western Blot analysis.RESULTS: Incubation with Vitexin for 48, 72 and 96 h was r esulted in a significant inhibition of SMMC-7721 cell proliferation i n a dose-and time-dependent manner(P<0.05), and IC50 values for t hem was 150.37 μmol·L-1, 116.24 μmol·L-1, 90.19 μmol·L-1, respecti vely. The rate of apoptotic SMMC-7721 cells was increased and ac companied by a decrease of the level of ?Ψm in a concentration-d ependent manner after treating with Vitexin for 72 h. The expression of P53, Bax, Casepase-3, PARP and other apoptosis-related genes were up-regulation and anti-apoptotic gene Bcl-2 was down-regulatio n in a concentration-dependent manner. The expression of p53, Bax,casepase-3, PARP and other apoptosis-related protein were up-regul ation and anti-apoptotic protein Bcl-2 was down-regulation in a con centration-dependent manner.CONCLUSIONS: Vitexin can potently disturb the proliferation of SMMC-7721 cells in vitro and induce apoptosis in a concentrat ion-time dependent, through mechanisms up-regulating the expressio n of Casepase-3, Bax, p53, PARP and down-regulating the expressi on of Bcl-2 by p53-dependent apoptotic pathway.